Lab 1-3 Flashcards
Name this organism
Protozoan- acanthamoeba
Name this organism
Euglena gracili (algae)
What solution is this
Hay infusion
Name this organism
Fungus : mold (aspergillus sp)
Name this organism
Fungus : yeast
Name this organism
Bacterium
What are the two most commonly used culture media?
nutrient broth and nutrient agar
What is the temperature at which agar melts? and solidify again?
melts at 90-100˚C and solidify at 40-50˚C.
What is a countable plate?
Plate that contains between 30-300 colonies
why wouldn’t you wet mounts to look at bacteria?
Because they don’t have much contrast with the surrounding liquid. They need to be stained
What is the primary disadvantage of the stainning?
kills the bacteria : no motility can be observed
What are the two organisms seen in wet mounts in the lab#3
euglena gradis and A.serpen.
Name this organism
pseudomona. aeruginosa
Name this organism
Staphylococcus aureus
Name this organism
aquaspirillum serpens
Name if the following are gram positive or gram negative :
- P.aeruginosa
- S. Aureus
- Tooth scraping cells
- gram negative
- gram positive
- gram positive
What are the steps of gram staining
What is ubiquity of microorganism?
presence everywhere or in many places especially simultaneously : omnipresence.
