Lab 1-3

Question 1

Name this organism

Answer 1

Protozoan- acanthamoeba

Question 2

Name this organism

Answer 2

Euglena gracili (algae)

Question 3

What solution is this

Answer 3

Hay infusion

Question 4

Name this organism

Answer 4

Fungus : mold (aspergillus sp)

Question 5

Name this organism

Answer 5

Fungus : yeast

Question 6

Name this organism

Answer 6

Bacterium

Question 7

What are the two most commonly used culture media?

Answer 7

nutrient broth and nutrient agar

Question 8

What is the temperature at which agar melts? and solidify again?

Answer 8

melts at 90-100˚C and solidify at 40-50˚C.

Question 9

What is a countable plate?

Answer 9

Plate that contains between 30-300 colonies

Question 10

why wouldn’t you wet mounts to look at bacteria?

Answer 10

Because they don’t have much contrast with the surrounding liquid. They need to be stained

Question 11

What is the primary disadvantage of the stainning?

Answer 11

kills the bacteria : no motility can be observed

Question 12

What are the two organisms seen in wet mounts in the lab#3

Answer 12

euglena gradis and A.serpen.

Question 13

Name this organism

Answer 13

pseudomona. aeruginosa

Question 14

Name this organism

Answer 14

Staphylococcus aureus

Question 15

Name this organism

Answer 15

aquaspirillum serpens

Question 16

Name if the following are gram positive or gram negative :

1. P.aeruginosa
2. S. Aureus
3. Tooth scraping cells

Answer 16

1. gram negative
2. gram positive
3. gram positive

Question 17

What are the steps of gram staining

Answer 17

Question 18

What is ubiquity of microorganism?

Answer 18

presence everywhere or in many places especially simultaneously : omnipresence.