Ionotrophic glutamate Flashcards
Types of glutamate receptor
AMPA
NMDA
Kainate
How many subunits make up all the glutamate receptor
- Can be heteromeric or homomeric
AMPA subunit names
GluA1
GluA2 (Q/R)
GluA3
GluA4
How many regions make up the AMPA subunit
M1-M4
AMPA: Which subunit region forms the pore element
M2
AMPA: Where in the subunits do the agonist bind
Extracellularly, between the D1 and D2 regions. So a total of 4 glutamate can bind to 1 receptor
Calcium permeable AMPA R lack which of the subunits
GluA2
What makes the AMPA receptor calcium impermeable
Presence of GluA2 subunit
What ions do glutamate receptors let in
CI AMPA: Na/K
CP AMPA: Na/K/Ca
NMDA: Ca especially, also Na and K
What is the RT time course for AMPA and NMDA
AMPA: 1ms RT
NMDA: 10ms
What is the decay time for AMPA and NMDA
AMPA: 3ms
NMDA: 100ms
How is a GluA2 cause AMPA to be CI
RNA editing at Q/R site in M2 region swaps glutamine for argenine, which has a positive charge
What type, CI or CP are inwardly rectifying
CP. Allows flow at resting potential
What can plug CP AMPAR
Polyamine in depolarised cells. Polyamines are positively charged, and moves towards the outside negative charge
NMDA structure
4 subunits.
GluN1 family (8 splice variants)
GluN2A, GluN2, N3, N4
GluN3A, GluN3B
