Investigating antibiotics and antiseptics Flashcards
Name the growth medium for bacteria
agar jelly
How to make agar jelly plate.
- pour hot agar jelly into shallow round petri dish
- once jellys cooled and set ,inoculating loop used to transfer microorganisms to agar j
- spreader can be used to get an even covering of bacteria
- Bacteria then multiply
How can we investigate effect of substances on bacterial growth.
place paper discs soaked in diff types of antibiotics
on it ,leave space between each one and a control one too
anti.. shud diffuse thro jelly ,antibiotic resistant ones continue to grow ,non resistant ones die =clear area inhibition zone
Explain what an aseptic technique is and why we use it ?
Name some aseptic techniques
creating a sterile environment /using equipment to prevent contamination (unwanted microorganisms )to affect your results
ethanol on bench kill microorganisms
pass inoculating loop thro hot flame-sterile
covered with lid lightly taped to prevent microorganism from air getting in
Calculate the size of inhibition zone
circle= pie r squared
measure in mm
Explain what a risk factor is
things linked to an increase in the likelihood of a person developing a certain disease
Name 2 unavoidable risk factors and 3 avoidable risk factors.
age ,gender
smoking -nicotine +heart rate + blood pressure damages artery walls ,build up fatty acids diet-too less malnutrition high in fat /sugar =risk factor obesity lack of exercise-risk factor of o alcohol-liver damage/disease
Name some economic and social effects of non-communicable diseases.
areas with high level of obesity,excess alcohol consumption,smoking,high occurrence of certain non-com..
put pressure on resources and hospitals
costly NHS provide resources,people with non…may not be able to work- effect economy
How to measures obesity
is it reliable?
BMI body mass index weight in kg/height m 2 not always bc athletes have muscle heavier than fat come out with high BMI,even if not obese below 18.5 underweight 18.5-24.9-normal 25-29.9-overweight 30-40-moderately obese above 40 severely obese
What is the waist-to-hip ratio
waist circumference cm/hip circumference cm
higher it is more weight around middle
above 1.0 males= too much weight
above 0.85=females
abdominal obesity greater risk of developing type 2 diabetes