Introduction to microbiology

Question 1

5 possible infecting agents

Answer 1

Bacteria, virus, fungi, parasite, prions

Question 2

4 Sterile sights

Answer 2

Blood, lung, bladder, CSF

Question 3

4 Non sterile sights

Answer 3

Nasopharynx, skin, urethra, gut

Question 4

What 2 things influence the sample taken

Answer 4

• Infection site

- Probable organism

Question 5

Sample for tonsilitis

Answer 5

throat swab

Question 6

Sample for meningitis

Answer 6

CSF

Question 7

Sample of a wound

Answer 7

Swab or pus

Question 8

Sample for chest problems

Answer 8

Sputum

Question 9

Urinary tract sample

Answer 9

Mid stream urine sample

Question 10

Diarrhoea sample

Answer 10

Faeces

Question 11

Bacteraemia sample

Answer 11

Blood culture

Question 12

Is microscopy sensitive or non sensitive?

Answer 12

Non sensitive

Question 13

What do not require use of a stain in microscopy

Answer 13

Parasite or pus

Question 14

Can microscopy identify the pathogen?

Answer 14

No

Question 15

Is culture sensitive?

Answer 15

Yes

Question 16

Non selective agar

Answer 16

Warmed up blood = chocolate

For mycobacteria

Question 17

Selective agar

Answer 17

Macconkey

Question 18

3 Ways to identify the particular species

Answer 18

• Observable characteristics eg pattern of growth
• DNA based tests
• Typing

Question 19

4 ways to Identify virus

Answer 19

• PCR
• Antigen detection
• Serology
• Electron microscopy

Question 20

3 Parasite groups

Answer 20

• Arthropod
• Helminth
• Protozoa

Question 21

Why is it hard to culture parasites?

Answer 21

Complex life cycle

Question 22

How do we detect parasites?

Answer 22

• Microscopy to identify stages ( P,O,C)

- Serology