Immunoassay and lab selection Flashcards
What are the assay types/detection methods?
Colourimetric - colour change
Turbidometric - cloudiness
Fluorometric - light excitement
Immunoassay - antigen/antibody binding, ELISA/RIA
How can colourimetry be used in detection?
Colour change can be related to the conc of the substrate of a reaction
Absorbance of light of a specific wavelenght can be used to calculate conc of a substrate
what are the components of an immunoassay?
Antibodies from animal (polyclonal or monoclonal) that react with hormone in species of interest
Tracer - enzyme or radioactive tag on molecule or antibody
Detection systems - colour, light, radiation
Separation - separate tracer signals that have reacted with hormone from those that haven’t
Describe the process of an ELISA test
Describe an RIA test
what are the advantages of an RIA detection system?
Advantage: not a lot can interfere with radiation
Disadvantage: need specialist facility
What are the advantages and disadvantages of ELISA detection systems
Advantages:
- avoid complexities of handling radiation
- common technology in-clinic
- no specialist required
Disadvantages:
- things can interfere with enzymes or light
What is chemiluminescence detection system?
Enzyme induced light emission rather than colour change
Common in non-specialist reference labs
What is validation and its components?
Validation = check the test can give correct results
Imprecision = how closely will the results match if we keep repeating the test
Accuracy = how far away from the real result is the tested result
What are the components of the packaging for blood tests - UN3373 - P650?
<50ml
3 components:
- primary receptacle
- secondary packaging
- outer packing
Extra info:
- leak proof
- secondary and outer packing rigid
- absorbent material between primary and secondary layers
