Immuno 5

Question 1

What cell surface markers are on B cells?

Answer 1

CD19, CD20, CD21, IgM, IgD

Question 2

What organelle is important in plasma cells?

Answer 2

RER bc it makes lots of protein (ie. immunoglobulin)

Question 3

What cell surface proteins are on T cells and what do they bind to?

Answer 3

TCR (antigen-MHC complex), CD3 (needed for TCR signal transduction), CD28 (binds B7 on APC)

Question 4

What cell surface proteins are on Helper T cells?

Answer 4

CD4, CD40 Ligand, CD3, TCR

Question 5

What cell surface proteins are on cytotoxic T cells?

Answer 5

CD8, CD3, TCR

Question 6

What cell surface proteins are on B cells?

Answer 6

Ig (binds antigen). CD19, CD20, CD21 (receptor for EBV), CD40. MHC II, B7

Question 7

What cell surface proteins are on MO?

Answer 7

CD14, CD40, CD16 (bind Fc). MHCII and B7. Fc and C3b (both enhance phagocytosis-ie. opsonins)

Question 8

What are cell surface proteins on NK cells?

Answer 8

CD16 (bind Fc of IgG) and CD56 (unique)

Question 9

What are the 5 types of heavy chain and what do they correspond to?

Answer 9

Delta-IgD. Mu-IgM. Gamma-IgG. Alpha-IgA. Epsilon-IgE.

Question 10

What are the types of light chains? Normal ratio?

Answer 10

Lambda and kappa. 2 kappa:1 lambda

Question 11

Function of the Fab?

Answer 11

Antigen-binding grag. Determines idiotype-unique antigen-binding porkcet; only 1 antigenic specificity expressed per B cell

Question 12

Function of Fc region?

Answer 12

Constant. Carboxy terminal. Complement binding at CH2. Carbohydrate side chain. Determinds isotype (IgM, IgD, etc.)

Question 13

What type of bonds are important in antibodies?

Answer 13

Disulfide bonds

Question 14

Functions of antibodies?

Answer 14

Opsonizaiton. Neutralization. Complement activation

Question 15

How does VDJ recombination occur?

Answer 15

Begins with breaks in the dsDNA at the recomination signal sequence (RSS) that flank the V, D, and J coding region. Then Recombination Activation gene complex (RAG 1 and RAG 2) recognize the RSS and initiate VDJ recombination.

Question 16

Oganelles important to plasma cells?

Answer 16

RER and Golgi apparatus

Question 17

Properties of IgG?

Answer 17

Most abundant Ab, In DELAYED response. Fixes complement, crosses placenta (provides passive immunity), opsonizes bacteria, neutralizes bacterial toxins and viruses. T1/2=21 days. Distrubuted evenly bw intravascular and extravascular pools.

Question 18

Properties of IgA?

Answer 18

Protects mucous membranes (MALT). Monomer in circ, dimer when secreted. Cross epithelial cells via transcytosis. Found in secretions (tears, saliva, mucus) and early breast milk.

Question 19

Properties of IgM?

Answer 19

Produced in the IMMEDIATE response to antigen.. Fixes complement, does not cross placenta. Monomer on B cells or pentamer in circ.

Question 20

Properties of IgD?

Answer 20

Found on surface of many B cells

Question 21

Properties of IgE?

Answer 21

Binds mast cells and baosphils. Cross-links when exposed to allergen, mediates HSN I rxn. Mediates immunity to prasitic worms by activating eosinophils. “allergy, eosinophils, IgE”