Hookworm Flashcards
MOT of Hookworms
Skin penetration
They have hooks used to attach to the host via their buccal cavity which has hooks or cutting plate which aids them in feeding
called the vampires because of the hook (ngipin)
Infective Stage of Hookworms
Filariform L3 Larva
Diagnostic Stage of Hookworm
Egg (heavy infection)
Usually yung magkakasabay na infection is the mix infection of
Ascaris, Trichuris, and Hookworm
Of these three si Hookworm ang pinaka-konti because para dumami si Hookworm kailangan there is heavy infection
Habitat of Hookworm
Small intestine
the only nematodes that live in the large intestine
Trichuris and Enterobius
all live in the small intestine
Ascaris, Hookworm, and Strongyloides
an animal hookworm
Ancylostoma brasiliense
Ancylostoma caninum
Old-World Hookworm
Ancylostoma duodenale
C-shaped structure (both sexes) is a unique characteristic of
Ancylostoma brasiliense
has fan-like structure in the tail called
copulatory bursa
used for copulation, and it is a trapping structure for the female
copulatory bursa
there is a spicule in the copulatory bursa used for penetration
one way to differentiate hookworms from one another is through their
buccal cavity
dental pattern of Ancylostoma duodenale
two (2) pairs of teeth
direction of tail and head are similar (whether towards the right or left)
Ancylostoma duodenale
bursa is described as bell-shaped
Ancylostoma duodenale
Cat hookworm
Ancylostoma braziliense
Canine/Dog hookworm
Ancylostoma caninum
TRUE OR FALSE
Ancylostoma spp. can also undergo
infection through ingestion of IS
TRUE
Intermediate host of the Hookworm
Small intestine
What are the distinct features that make them
hookworms?
Anterior end are all curved / hook mucosa to attach
themselves to the intestinal mucosa
forms a conspicuous bend at the anterior portion of
the parasite
Hookworm
TRUE OR FALSE
The hookworm egg cannot be distinguished whether
it is Ancylostoma duodenale, Necator americanus,
Ancylostoma braziliense, Ancylostoma caninum
TRUE
Hookworm egg can be mistaken as
Strongyloides egg
○ Capable of infecting man
○ The final host would be the cat
Ancylostoma braziliense
○ Capable of infecting man
○ Final host is k9, mga aso
Ancylostoma caninum
New world hookworm or American
murderer (common in the Philippines)
Necator americanus
A. duodenale:
Filariform larva / L3 is characterized as
pointed tail, sheathed, shorter
esophagus
Hookworm egg characteristics
● Thin shelled
● 2 - 8 cell stages (blastomeres)
● No species identification
stage of hookworm wherein
open mouth
stage, it has long buccal cavity with small genital
primordium
Rhabditiform L1
DEntal pattern of Necator americanus
Semilunar cutting plate
“c” shaped:
”s” shaped:
“c” shaped: A. duodenale
”s” shaped: N. americanus
Dorsal ray,
trigiditate or
“tripartite” is seen in
A. duodenale
Dorsal ray, bidigate
or “bifid is seen in
N. americanus
● Larval migration through lungs
N. americanus
A. duodenale
Diagnostic stage of A. duodenale and N. americanus
Thin-shelled eggs (4-16 or blastomere stage)
2-8 cell stages (packet)
MOT of:
A. duodenale:
N. Americanus:
A. duodenale: Skin penetration/percutaneous AND ingestion
N. Americanus: ONLY skin penetration / percutaneous
Life span of A. duodenale and N. americanus
Up to 15 yr or more ; usually 5-10 years
TRUE OR FALSE:
You can differentiate the eggs of A. duodenale from N. americanus
FALSE
Can only differentiate adult
but the eggs and the larva, you cannot
distinguish which hookworm is that
TRUE OR FALSE:
You can differentiate the larvae of hookworm larvae from Strongyloides larvae
TRUE
we can distinguish kung ano doon yung
hookworm larva vs strongyloides larvae but their eggs are
the same
With 2 ventral pairs of teeth both found in female and
male hookworm
ANCYLOSTOMA DUODENALE
Spicule of Ancylostoma duodenale
plain and bristle-like
Spicule of Necator americanus
Barbed, fused
NEcator americanus doesn’t have real teeth but have
semilunar cutting plates
Feeding stage
Rhabditiform L1
Pathology of Human Hookworms
Feel itch in the place where it entered
Cutaneous Stage: Ground Itch
Pathology of Human Hookworms
Can undergo lung larval migration
Ascaris lumbricoides and Hookworms
Pulmonary Phase: Pneumonitis
Pathology of Human Hookworms
Blood loss because it lives in the blood
Intestinal: Blood Loss; Iron Deficiency Anemia
2 Pathologies of Animal Hookworms
Cutaneous Larval Migrans / Creeping eruption;
Eosinophilic Enteritis
Wakana/katayama disease is caused by ____ and is manifested by ____
Hookworm
Headache, Chills, Fever, Eosinophilia and Diarrhea
altogether
Can indicate inflammation, allergic reaction, or
parasitic infection if more than __% eosinophilia
70%
Adult hookworms can cause anemia by which RBCs are
RBCs are microcytic hypochromic
Food of hookworm
Blood
Smallest nematode
STRONGYLOIDES STERCORALIS
Demonstrates Parthenogenesis
Can fertilize its own egg
Does not require the male for fertilization
STRONGYLOIDES STERCORALIS
Difference of male and female strongyloides stercoralis
Female - pathogenic
Male - free living
Also called Thread Worm
STRONGYLOIDES STERCORALIS
Habitat of Strongyloides stercoralis
small intestine
Infective stage of stronglyloides stercoralis
Filariform larva L3
MOT of stronglyloides stercoralis
Larval Penetration/Skin Penetration
Unique characteristic of Strongyloides stercoralis rhabditiform
PROMINENT genital primordium
Difference between Hookworm rhabditiform and S. stercoralis rhabditiform
Hookworm rhabditiform: small genital primordium
S. stercoralis rhabditiform: prominent genital primordium
Difference between Hookworm filariform larva and S. stercoralis filariform larva
Hookworm L3 : Shorter esophagus ; pointed tail
S. stercoralis L3: Longer esophagus ; notched tailq
One of the parasites that cause severe diarrhea
STRONGYLOIDES STERCORALIS
Types of diarrhea caused by S. stercoralis
Vietnam Diarrhea
Cochin China Diarrhea
Honeyform appearance of intestinal mucosa is one of the pathologies of
S. stercoralis
Capable of Larva Currens – migration of the larva
S. stercoralis
Baermann Technique (Culture technique)
Funnel Rubber tubings Clap Gauze / Wire screen Water (Moisturized water)
Principle of Baermann Technique (Culture technique)
Based on the active migration or movement of larvae
➢ They sink to the bottom and can be collected for
identification
What stage is harvested in Baermann technique
Filariform larvae
this is known as “Filter paper culture technique”
Harada Mori (culture technique)
Harada Mori (culture technique) conditions
Dark place
7 days
25 degrees (cold)
What stage is harvested in Harada Mori Technique
Filariform larvae
TRUE OR FALSE
Harada Mori TEchnique and Baermann technique are used in clinical setting for diagnosis
FALSE
Harada Mori TEchnique and Baermann technique are only used for RESEARCH PURPOSES
very important in detecting light
infection with hookworms, Strongy, Trichostrongylus
species.
Harada Mori TEchnique
a good method for culturing Strongyloides
stercoralis.
Agar plate culture
consists of agar, meat extract, peptone, and
saline solution.
Agar plate culture
Can you differentiate Ancylostoma
duodenale vs. N. americanus using this egg?
NO
Can you distinguish the Hookworm vs. the
Strongyloides by simply observing this egg?
NO
What is the feature of the Strongyloides egg that can
be observed but not enough to differentiate it from the
hookworm eggs?
The size. The Strongyloides egg is slightly smaller.
body cavities of nematodes are
pseudocoelom
Nematodes are one of the largest groups of
metazoan
Nematodes Bilaterally symmetrical cylindrical body with
tripoblastic nature
Body of nematodes is
slender cylindrical unsegmented
with a resistant cuticle
Nematodes Body wall contains
longitudinal muscles
Circular muscles are absent
NEMATODES
Epidermis is ____
Locomotory cilia are ____
syncytial.
Absent but
Some have ciliated gastrodermal cells
NEMATODES
Alimentary canal is a
simple straight tube.
NEMATODES
Digestion is both
extracellular and intracellular
TRUE OR FALSE
Nematodes’ Intestine is non-muscular.
TRUE
TRUE OR FALSE
Nematodes have Blood vascular system
FALSE
Blood vascular system is absent
Pseudocoelomic Fluid helps in the transport of nutrients.
NEMATODES
Excretory system contains
‘H’ shaped canals or excretory
gland.
NEMATODES
Respiration is by
anaerobic method.
TRUE OR FALSE
NEMATODES
Nervous system consists of a circum-enteric nerve ring
with ganglia.
TRUE
Sense organs are aphids and phasmids
glandulo sensory structures in the posterior region of the
body.
(mechanical and chemoreceptors
present at the anterior region of the body)
NEMATODES
Sexes are separate. Sexual dimorphism is present.
TRUE
TRUE OR FALSE
Fertilization is internal and external
FALSE
Fertilization is INTERNAL
Development involves how many moults of the cuticle
four
It is a phenomenon in which
cell division ceases near the end of embryonic
development, so that the number of cells of the adult is
constant. Development of rhabditiform larva. THIS IS CALLED
EUTELY
2 CLASSIFICATION OF NEMATODA:
Class 1: Aphasmidia
Class 2: Phasmidia
➢ These are mostly free living.
➢ Amphids are of various shapes and highly
modified.
➢ Phasmids are absent
➢ Excretory glands are present and excretory
canals are absent.
Aphasmidia
Some are freeliving and others are parasitic
➢ Amphids are porelike.
➢ Phasmids are present.
➢ Excretory glands / excretory canals or both are
present.
➢ Examples: Ascaris common round warm,
Enterobius pinworm, Wuchereria Filarial worm,
Ancylostoma hookworm.
Phasmidia
Flattened on one
side
Ascaris
. Obvious
albuminous coating
Ancylostoma
Surrounded by a
nurse cell
Enterobius
Thin shell
surrounding
embryonic cleavage
Trichuris
Polar plug on each
end
Trichuris??