Histology: Connective Tissue (lab)

Question 1

What cells appear granular and blue as a result of ferritin injection and prussian blue die?

Answer 1

Macrophages

Question 2

Compare loose connective tissue to dense connective tissue?

Answer 2

typically has more cells and less ECM

Question 3

These cells have elongated nuclei that are typically larger and paler than that of macrophages?

Answer 3

Fibroblasts

Question 4

Compare the nuclei of mesothelial cells to Fibroblast nuclei?

Answer 4

These are larger and more pale than fibroblasts

Question 5

What cells appear red and are full of naturally occuring granules?

Answer 5

Mast cells

Question 6

What cells have spotty looking nuclei with 3 to 5 lobes?

Answer 6

Granulocytes/Neutrophils

Question 7

What makes up the mononuclear phagocytic system throughout the body?

Answer 7

Macrophages

Question 8

What is the job of macrophages?

Answer 8

clean up of debris
Remodeling after injury
monitoring of the body through the innate immune system

Question 9

T or F: Macrophages have antigens presented on their surface to the adaptive immune system by membrane proteins?

Answer 9

True

Question 10

What are the types of membrane proteins presented on the macrophage?

Answer 10

major histocompatibility complex (MHC) or human leukocyte antigen (HLA)

Question 11

When do macrophages become activated?

Answer 11

by injection or infection by foreign substances

Question 12

What are macrophages in the hepatic sinusoids called?

Answer 12

Kupffer cells

Question 13

T or F: in healthy mesentery tissue a large number of macrophages will be present?

Answer 13

False

Question 14

What tissues typically have a larger number of macrophages?

Answer 14

Hepatic sinusiods, Alveolar walls, and Red pulp of the spleen

Question 15

What cells are macrophages derived from?

Answer 15

Monocytes

Question 16

What is a characteristic of macrophages in the lungs?

Answer 16

They might appear black because of carbon in the cytoplasm

Question 17

In skin the nuclei around dense green collagen fibers are most likely….

Answer 17

Fibroblasts

Question 18

Where are plasma cells commonly found?

Answer 18

Under the epithelium in G.I. tract, Bone marrow, and respiratory tract

Question 19

What are the distinguishing features of the plasma cell?

Answer 19

round nucleus (off center), Heterochromatin clumped like a clock face, pyramid cytoplasm, basophilic cytoplasm

Question 20

What color does collagen appear in H & E?

Answer 20

pink, sometimes hard to tell from smooth muscle

Question 21

How do elastic fibers appear in H & E?

Answer 21

Thin dark streaks, while collagen is thicker and lighter (in unsectioned tissue)

Question 22

What color stain is used to distinguish elastic tissue from collagen?

Answer 22

dark purple

Question 23

What kind of type of fibers are often visualized using silver staining?

Answer 23

Reticular fibers (appear as black threads)

Question 24

Where are reticular fibers common?

Answer 24

Stroma of the lymph node

Question 25

What type of connective tissue predominates in mesentery?

Answer 25

Loose connective tissue - lots of space between collagen fibers

Question 26

Where can dense irregular connective tissue often be found?

Answer 26

Surface of the skin, capsules of organs

Question 27

Where is dense regular connective tissue often found?

Answer 27

Muscles, tendons, aponeurosis

Question 28

What are the two components of tendon?

Answer 28

fibroblasts, collagen

Question 29

What type of fiber secreting cells can be found within a lymph sinus with H&E stain?

Answer 29

reticular cells - elongated nucleus

Question 30

T or F: reticular cells can be visualized with silver stain?

Answer 30

False - reticular fibers CAN be visualized with silver stain

Question 31

What type of cell looks like chicken wire?

Answer 31

Adipose

Question 32

Why are the nucleus and cytoplasm pushed against the side of fat cells?

Answer 32

Because triglycerides take up the majority of the space

Question 33

What types of cells are stained black by osmium?

Answer 33

myelin (black rings) , Fat (black blobs)