Histology: Connective Tissue (lab) Flashcards
What cells appear granular and blue as a result of ferritin injection and prussian blue die?
Macrophages
Compare loose connective tissue to dense connective tissue?
typically has more cells and less ECM
These cells have elongated nuclei that are typically larger and paler than that of macrophages?
Fibroblasts
Compare the nuclei of mesothelial cells to Fibroblast nuclei?
These are larger and more pale than fibroblasts
What cells appear red and are full of naturally occuring granules?
Mast cells
What cells have spotty looking nuclei with 3 to 5 lobes?
Granulocytes/Neutrophils
What makes up the mononuclear phagocytic system throughout the body?
Macrophages
What is the job of macrophages?
clean up of debris
Remodeling after injury
monitoring of the body through the innate immune system
T or F: Macrophages have antigens presented on their surface to the adaptive immune system by membrane proteins?
True
What are the types of membrane proteins presented on the macrophage?
major histocompatibility complex (MHC) or human leukocyte antigen (HLA)
When do macrophages become activated?
by injection or infection by foreign substances
What are macrophages in the hepatic sinusoids called?
Kupffer cells
T or F: in healthy mesentery tissue a large number of macrophages will be present?
False
What tissues typically have a larger number of macrophages?
Hepatic sinusiods, Alveolar walls, and Red pulp of the spleen
What cells are macrophages derived from?
Monocytes
What is a characteristic of macrophages in the lungs?
They might appear black because of carbon in the cytoplasm
In skin the nuclei around dense green collagen fibers are most likely….
Fibroblasts
Where are plasma cells commonly found?
Under the epithelium in G.I. tract, Bone marrow, and respiratory tract
What are the distinguishing features of the plasma cell?
round nucleus (off center), Heterochromatin clumped like a clock face, pyramid cytoplasm, basophilic cytoplasm
What color does collagen appear in H & E?
pink, sometimes hard to tell from smooth muscle
How do elastic fibers appear in H & E?
Thin dark streaks, while collagen is thicker and lighter (in unsectioned tissue)
What color stain is used to distinguish elastic tissue from collagen?
dark purple
What kind of type of fibers are often visualized using silver staining?
Reticular fibers (appear as black threads)
Where are reticular fibers common?
Stroma of the lymph node
What type of connective tissue predominates in mesentery?
Loose connective tissue - lots of space between collagen fibers
Where can dense irregular connective tissue often be found?
Surface of the skin, capsules of organs
Where is dense regular connective tissue often found?
Muscles, tendons, aponeurosis
What are the two components of tendon?
fibroblasts, collagen
What type of fiber secreting cells can be found within a lymph sinus with H&E stain?
reticular cells - elongated nucleus
T or F: reticular cells can be visualized with silver stain?
False - reticular fibers CAN be visualized with silver stain
What type of cell looks like chicken wire?
Adipose
Why are the nucleus and cytoplasm pushed against the side of fat cells?
Because triglycerides take up the majority of the space
What types of cells are stained black by osmium?
myelin (black rings) , Fat (black blobs)
