HISTO LAB 1 LESSON 1 Flashcards
Comes from the Greek word “_____” and
“_____” which means “small” and “to
look”
mikros and skopein
Used to see objects that are too small for the
naked eye
microscope
Shape, size, position, connections, colors,
number, texture, and even chemical
composition.
microscope
is to get a magnified image,
in which structures may be resolved which could
not be resolved with the help of an unaided eye.
Microscopy
The ratio of the size of an object seen under a microscope to the actual size observed with unaided eye. o The object appears larger under the microscope
MAGNIFICATION
Ability to distinguish two objects
from each other.
RESOLUTION
The distance through which you can
move the specimen and still have it
in focus.
DEPTH OF FIELD
Carries the optical part of the
microscope.
HEAD
o Connects the head and the base
o Part where we hold/carry the
microscope from one place to
another
ARM
o Support of the microscope
o Carry the light source of the
microscope
BASE
o Support of the microscope
o Carry the light source of the
microscope
BASE
a. Part where we look through the microscope b. Found on top c. Standard magnification power of each eyepiece is 10x.
Ocular Lens
a. Can be move to adjust and have a
clearer magnification view of the
specimen.
Diopter Adjustment
4 types of objective lens
a. Scanner
b. LPO
c. HPO
d. Oil Immersion
3 major parts of microscope
head, arm, and base
a. Hold the objective lens
b. Can be revolve
Nose piece
a. Course, bigger movement; move the
microscope up and down
b. Fine, smaller movement;
Adjustment knobs
a. Specimen is place here for viewing
Stage
a. Holds the specimen in place
Stage clip
a. Used to move the stage the up and
down, left and right.
Stage controls
a. A hole on the microscope stage
through which the transmitted light
from the illuminator will reach on
the stage.
Aperture
a. Located at the base
b. Captures the light from the external
source or the voltage (electric
microscope) to have a focus life unto
the specimen.
. Light source
a. Found under the stage, next to the diaphragm b. Ensure clear sharp images c. Without it, image will not be as sharp as what we going to observe under the microscope.
Condenser
a. Found under the stage as well
b. Control the amount of light from the
light source that will pass through to
the specimen.
. Diaphragm
→ Always carry your microscope with two hands,
one grasping the arm or back slot and the
other supporting the base.
→ Left- hold the base
→ Right- hold the arm of the microscope
CARRYING:
→ Turn off light & center mechanical stage
→ Position the nosepiece so that the lowest
scanning (4x) objective is in place.
→ Remove the slide from the stage and put it in
the proper place.
→ Clean the stage and lenses with gauze and
lens cleaner and wipe off any oil.
→ Wrap the cord around the arm.
→ Carefully carry with two hands and gently
place the microscope in the proper cabinet.
PUTTING AWAY:
→ Set the microscope on a flat, solid support
and in a position where it will not easily be
knocked off.
→ Properly coil the cord to avoid tripping over
it.
TABLE PLACEMENT:
→ Lenses must be clean for resolution.
→ Use only lens paper or gauze and cleaning
solution.
→ Never use your finger, handkerchief, paper
towels or spit to clean the lenses.
→ Do not remove any parts for cleaning; it only
allows dust to enter the microscope.
CLEANING
____ are the building blocks of all living things.
cells
such as those of plants and
animals, are structurally complex.
Eukaryotic cells
o Have nucleus and separated from the cytoplasm of the nuclear envelope o Cytoplasm contains membrane organelles: ribosomes, cytoskeleton o Have cell wall on the surface and have undergone the process of differentiation and specialization. o Compose of tissues and organize to organ-to-organ system.
Eukaryotic cells
such as those bacteria, have
a simple organization.
Prokaryotic cells
o Single cellular DNA which is not separated form the cytoplasm by membrane o Do not have any membrane organelles o Small ribosomes are present o Have cell wall on the surface o Reproduce thorough asexual reproduction or by amitosis or binary fission which occurs immediately after DNA replication.
Prokaryotic cells
, as if the family tree of life on Earth
split into three main branches, called domains
eukarya
bacteria
Archaea
: Plants, animals, fungi, and plants
eukarya
Familiar, single-celled
microorganisms, some of which are useful to
humans and some of which cause human
disease.
BACTERIA
Single-celled microorganisms found
in all types of environments but first
discovered in extreme environments, such as
hot spring.
archaea
Locate the Acinar cells
and the islets of Langerhans
pancreas
hold the base
Left
hold the arm of the microscope
Right
contains membrane organelles: ribosome, cytoskeleton
Cytoplasm
The microscope was invented by _________
Anton Van Leeuwenhoek
Microscope is an important tool for ____
Medtech
Microscopes part and functions has been perfected over the past ____ years since it is invented
300 years
