Hepadnaviridae & Retroviridae Flashcards
What group are hepadnaviridae in?
Group 7–>dsDNA w/ RNA int
True or false: Hepadnaviridae do not need to bring in their own DNA pol
False
-Need to bring in VIRAL RNA dep DNA pol (REVERSE TRANSCRIPTASE)
What pathogens are in Hepadnaviridae
Hepatitis B
Why is Hepatitis B concerning?
-Common–>1/3 of population has gotten it
-90% clear infection, but 1% develop acute liver failure & die
-can develop life long chronic infection (worse in children)
-cirrhosis
-cancer
Differences between Hepatitis A, B, and C
Hep A: Picornaviridae–>Group 4 (+)ssRNA, mild infection, vaccine exists
Hep B: Hepadnaviridae, Group 7 dsDNA w/ RNA int, chronic infection, vaccine exists–>not available globally
Hep C: Flaviviridae, Group 4 (+)ssRNA, chronic, no vaccine
True or false: Hepatitis kills less people than malaria, tuberculosis, and HIV/AIDS
False
-Hepatitis has killed more in 2013
What family is Hepatitis E in?
Togaviridae
Which hepatitis are the biggest killer?
Hepatitis B and C
Is Hepadnaviridae enveloped or non, what type of capsid?
-Enveloped
-Icosahedral
Hepadnaviridae genome characteristics?
-rcDNA (relaxed circular DNA)–>strand is not complete/covalently linked, not circular
-1 DNA strand has viral pol at 5’ end
-Other strand has m7G capped RNA
Hepadnaviridae life cycle
- Entry–> fusion or endocytosis
- Becomes circular dsDNA –>Host DNA pol repairs rcDNA into cccDNA
- RNA transcription (host DNA dep RNA pol)–>5 protein coding mRNA, includes pgRNA
- Protein translation–> 7 proteins
- Encapsidation–>pgRNA is packed into capsids
- Genome synthesis–>made by VIRAL reverse transcriptase
- Budding and exocytosis
Once in the nucleus hepadnaviridae do what?
dsDNA becomes circular by cccDNA (covalently closed circular DNA)
What is an advantage rcDNA becoming cccDNA?
Circular genomes are more stable and can stay for decades
-b/c circular/episomal it is not recognized as non-self by host
How is pgRNA made and what does it do?
HOST RNA makes 5 RNAs that includes pgRNA
-used as a template for reverse transcriptase
True or false: pgRNAs and reverse transcriptase are packaged into virions after reverse transcriptase steps occur
False
-pgRNAs and reverse transcriptase are packaged into virions BEFORE RT steps
Reverse transcription steps for Hepadnaviridae
- RT binds to stem loop at 5’ end to start
- RT switches to identical seq at 3’ end
- DNA is made, RNA is degraded, but a little remains
- The RNA starts 2nd strand synthesis
- 2nd strand uses DNA as template so DNA dep DNA pol is used
- dNTPs are used up and synthesis stops making rcDNA (not circular)
True or false: Hepadnaviridae make many infectious particles
False
-Icosahedral capsid does not need nucleic acid to from so it forms many non-infectious particles
What are satellite virus and example of one
Parasitize the machinery of other viruses
-Hepatitis D