Hematology Flashcards

1
Q

hematopoiesis

A

blood cells continuously produce in the bone marrow
all blood cells arise from the stem cell pluripotent

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2
Q

blood functions

A

transport, protection, and regulation
O2/nutrient transport, clotting proteins, antibody and cellular protection against infection, waste removal through kidneys and liver, body temperature regulation

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3
Q

hematology

A

study of blood
blood is a tissue

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4
Q

why do we study hematology?

A

for diagnostic evaluation of disease and screening prior to surgery

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5
Q

what should be considered prior to blood draw?

A

which diagnostics are needed?, determine which blood collection tube should be used, figure out how you are going to collect (needles, syringes, butterfly catheters), determine blood vessel you are going to draw from (bigger vein flows quicker), will the patient need to fast beforehand?

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6
Q

larger needle does what to cells?

A

increases damage to cells

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7
Q

why do you need to watch syringe suction?

A

it can increase damage to cells

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8
Q

what should you prepare environment wise for blood draws?

A

make the environment as stress free as possible
tourniquet, towels, sedation, etc
stress can alter test results (especially in cats) and success of blood draw

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9
Q

how do you determine needle size for venipuncture?

A

needle size should match vein size

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10
Q

what are the different types of blood collection tubes?

A

anticoagulants and chemistries

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11
Q

anticoagulant blood tubes

A

purple top: EDTA
green top: Heparin
blue top: Citrate

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12
Q

chemistry tubes

A

red/tiger top: no additives, serum separator tube

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13
Q

what tests are used for each type of blood tube?

A

EDTA: CBC
Heparin: CBC and chemistry
Citrate: coagulation test

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14
Q

microtainers

A

holds 0.1-0.5 mL of blood

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15
Q

tips for taking a blood sample

A

never push blood through the needle, invert anticoagulant tubes immediately (don’t shake), refrigerate if not used within 1 hour, valid if used within 1 week

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16
Q

automated analysis

A

Abaxis and Idexx

17
Q

manual RBC/WBC count

A

counts total number of white and red blood cells in a unit of blood

18
Q

how are manual WBC/RBC counts done?

A

using a Hemocytometer, usually for exotics, estimate from blood smear

19
Q

in-house CBC advantages

A

advantages: earlier diagnosis, better pre-anesthesia management, minimization of artifacts

20
Q

in-house CBC disadvantages

A

expensive and take time, require technical skill, manual counts aren’t as accurate

21
Q

CBC

A

counting WBCs and RBCs
uses RBC indices which measure the size, shape, and quality of the RBCs
packed cell volume (PCV) and hematocrit (HCT)

22
Q

which of these tests are manual and which is on a machine?
-packed cell volume (PCV)
-hematocrit (HCT)

A

PCV is manual and HCT is on a machine

23
Q

what are some other blood tests that can be done?

A

differential/Hemogram, platelet estimate, total protein, and hemoglobin test

24
Q

peripheral blood smear

A
  1. drop of blood on slide
  2. use the edge of another slide to smear
  3. stain
25
Q

Diff-quick stain

A

fixative: Methanol
stain 1: eosinophilic (red)
stain 2: basophilic (blue)

26
Q

differential blood count test

A

used to evaluate the monolayer
distribution of 5 different WBCs
count 100 WBCs under oil
expressed in % and absolutes

27
Q

packed cell volume test PCV/HCT

A

measured as %
PCV: manual
HCT: automated, (MCV x RBC count) / 10

28
Q

total protein (TP) test

A

useful in determining hydration status
measured with a refractometer
normal range: 6.0-8.0 g/dL in most mammals (varies per species)
calibrate and clean with distilled water

29
Q

normal ranges for PCV test

A

dog: 35-57%
cat: 35-45%
horse: 27-43%