heamoproteins 1

Question 1

—in the rbc responsible for 02 transport in higher animals and is — of iron in our body

Answer 1

• haemoglobin
• 65%
• RMM: 66000

Question 2

— in muscles which stores and transports 02 across muscle cells and is — of the iron

Answer 2

• myoglobin
• 6%
• RMM: 17.8

Question 3

myoglobin is a — and it consist of — which contains — and —-

Answer 3

• tertiary structure
• haem group of: iron II and protoprophryin IX
• globin chain that is secondary and primary and has 153-160 amino acid residues

Question 4

—- refers to the linear sequence of amino acids in the polypeptide chain and are linked by — such as —

Answer 4

• primary structure
• covelant bond
• peptide bond

Question 5

— refers to highly regular local sub structure and consist of two main types — and —. These structures are defined by patterns of —- between the main-chain peptide groups

Answer 5

• secondary
• alpha helix , beta sheeted
• hydrogen bonding

Question 6

— refers to the 3d structure of protein. The alpha helixes are beta sheetes are folded into — structure. The interactions include ——–

Answer 6

• tertiary structure
• globular
• salt bridges, disulfied, hydrogen bonds, and tightly packed side chains

Question 7

— 3d structure of multi-subunit proteins and the subunits fit together. These structures are stabilised by —– and —–

Answer 7

• quantery
• non covalent binds
• disulfied interactions as tertiary structure

Question 8

iron - protoprophryin IX is in — family and has —- atoms and is —- with — pyrrole like rings joined by — and is —–

Answer 8

• porphyrin , tetradentate, planer ligands
• 4N donor atoms
• hetroaromaric
• 4
• CH group
• peripheral substituents

Question 9

— has a compact shape with protein chain folded about the haem group.

Answer 9

myoglobin

Question 10

globin chain linked to the haem group through the histadine residue called

Answer 10

proximal histadine

Question 11

myoglobin has — helical and —-
the interior is —-
exterior is —-
and only 2 —— and both are essential for —-

Answer 11

• 8 helical regions
• non helical regions and termini chain
• hydrophobic
• hydrophilic
• hydrophilic
  -biological activituies

Question 12

the fe II is — and the coordination number is — and has — amount of maximum coordination number and has a – coodrination site to complex with —

Answer 12

• unsaturated
• 5
• less
• vacant
• 02

Question 13

the haem group is — or else it will be —- which can’t bind to 02

Answer 13

• non-polar , hydrophobic
• oxidised to fe III ( in the presence of 02 and h20 )

Question 14

the forms of myoglobin:
- myoglobin is — + – and the color is —
- oxymyoglobin is — + — and the color is —
- metomyoglin is —- + — and the color is —

Answer 14

• fe II + histadine , purple red
• fe II + histadine +02 , bright red
• fe III + histadine + h20 , brownish red and can’t bind to 02

Question 15

energies in the metal ion of – orbital in — and dependent on — and the two splits are — and — and the two possible configurations are

Answer 15

• d
• octahedral complex
• axial , inter axial
• high spin , low split ( paramagnetic, u fill singly )
• low spin , high split ( diamagnetic , u fill all down first)

Question 16

myoglobin has — spin and lies – of the prophrin plane and has – coordiantion side and coordination number is —
oxymyoghobin has — and is — in size and electrons are concentrated into – orbitals and they fit – the porphyrin plane and is — with coordination number —

Answer 16

• high spin , paramgantic , fe II
• out
• one empty
• 5
• low spin , diamagnetic , fe II
• smaller in size
• 3
• into
• saturated
• coordination number 6

Question 17

haemoglobin has – shape and is — the 3D structure of globin chain in Hb is similar to — although the amino acids are — at — position
consist of — subunits and are —like

Answer 17

• spherical
• non polar
• myoglobin
  -identical
  -24
  4 subunits 2 alpha and 2 beta w/ salt bridges in between
• myoglobin-like

Question 18

The adult human hemaoglobin (HBA ) is a — protein
- alpha has – amino acids
- beta has — amino acids
- each alpha is in contact w — beta
- very few interactions between — or —

Answer 18

• tetrametric
• 141
  146
• both betas
• 2 alphas or 2 betas

Question 19

the most important interactions between the subunits are

Answer 19

salt bridges linkages between oppositely charged amino acid side chains

Question 20

subunit in the haem group:
- each haem group is —
- fe II is — and the coordination number is —
- vacant coordination site to complex with —
- has – spin with – unpaired electrons
- Fe II is – of the plan

Answer 20

• Mb-like
• unsaturated , 5
• 02
• high spin , 4 unpaired electrons
  -out

Question 21

true or false: when iron II enters the porphyrin plane it shrinks

Answer 21

true

Question 22

the – structure of globin chain in haemoglobin is — from the myoglobin but the — and — are —. hemoglobins structure is — but myoglobin isn’t.

Answer 22

• primary
• different
• seoncdary and terirty
  -quantery

Question 23

when haemoglobin becomes oxyhemoglobin is called —
-formula:
-coordination number becomes:
- iron is – the plane
- – spin
- is the process reversible?

Answer 23

• oxygenation
• Hb + 402 –> <– ( aka equilibrium lol ) Hb(02)4
• 6 , saturated
• in
• low spin , diamagnetic
• yes