GLOSSARY Flashcards
hyphae that grows above or outward from the surface of an agar medium or substrate; the visible colony
aerial hyphae
Gelatinous substance used as a culture medium to grow microorganisms
Agar
Gelatinous substance used as a culture medium to grow microorganisms
Agar
polysaccharide from seaweed extract Genera Gelidium and Gracilaria) that consists of alternating BD-galactose and 3, 6-anhydro-L-galactose units of agarobiose. It is used popularly as a medium for electrophoresis to separate DNA fragments. In general, the higher its concentration the smaller the pore size. Concentration ranges from 0.5% - 2%
Agarose
asexual (imperfect) state or form of a fungus.
Anamorph
this a step in PCR when the temperature is lowered to allow DNA primers to attach to a given DNA template.
Annealing
Temp of annealing
50-56
fungus that preferentially grows on human rather than animals or the soil. A dermatophyte where human/people is the primary reservoir
Anthropophilic
conidium released by fragmentation or by separation that occurs at the septum of cells of hypha.
arthroconidium
fruiting body within which asci form
ascocarp
saclike cells where ascospores develop
ascus
hypha without crosswalls
aseptate
antimicrobial that can kill a microorganism
bactericidal
a virus that infects bacterial cells
Bacteriophage
An arrangement observed in Aspergillus spp. where a secondary phialide develops from a primary phialide, forming two series of phialides.
Biseriate
conidia formed by budding process along a hypha, pseudhypha, or in a single cell, as seen in yeasts.
Blastoconidia
nutrient-rich medium used to isolate fastidious fungi
Brain heart infusion
concentration of an antimicrobial that defines if the particular microbial species is susceptible or resistant to the antimicrobial.
breakpoint
liquid medium containing nutrients used to grow microorganisms
Broth
type of asexual reproduction commonly found in yeasts. An oval shaped “pushed outˮ or outgrowth from parent cell
Bud
colorless dye that binds to chitin and cellulose of fungi that fluoresce when exposed to ultraviolet light
Calcofluor white
proteinaceous coat that protects the viral genome
Capsid
hyaline mucopolysaccharide covering the cell body of certain yeasts
Capsule
Also known as chlamydospores, there are enlarged, thick walled round conidia. These conidia can be present at the end of the hypha (terminal), on the sides (sessile) or in between the hypha (intercalary)
chlamydoconidia
swollen, thick-walled resistant spore that do not reproduce
chlamydospore
club-shaped
Clavate
hyphal structures without septa
coenocytic
remnant of a cell wall present at the tip of a phialide, or around a sporangiophore
Collarette
growth of an organism in a host without causing infection or tissue invasion
Colonization
distinct characteristics of a microbial colony that are visibly observed on the surface of a nutrient medium
Colony morphology
sterile dome-like structure at the tip of a sporangiophore or within a sporangium.
columella (pl. columellae) -
serologic procedure used to determine complement fixing antibody to fungal antigens
complement fixation
pertains to a type of growth on the agar surface wherein it is continuous, uniform, and the colonies are not discrete or separated; there should be no individual colonies growing on the surface.
confluent -
cell that gives rise to a conidium.
conidiogenous cell -
reproductive propagule produced in the absence of nuclear recombination, thus representing anamorphic or asexual reproduction.
conidium (pl. conidia)
specialized hypha that gives rise to or bears a conidium.
conidiophore
method of propagating microorganisms by supporting their growth using culture media and controlled environmental conditions
cultivation
distinct visible visual changes in the infected cells caused by a viral toxin or infection
cytopathic effect
Distinct visible visual changes in the infected cells caused by a viral toxin or infection
cytopathic effect
an infection of cutaneous tissue (hair, skin, and nails) caused by the keratinophilic fungi other than those of the dermatophytes
Dermatomycosis
a fungi that invades the keratinized part of the hair, nail, and skin.
dermatophyte
a fungus having brown or black melanotic pigment in the cell wall.
dematiaceous
this initial stage in PCR allows separation of the double-stranded DNA into two single strands by breaking the hydrogen bonds that exists between the nucleotide base pairs
Denaturation
Temp ranges from 94-95
Denaturation
fungi having two morphologic form under different growth conditions
Dimorphic
elimination or reduction of microorganisms that may cause disease.
disinfection
mou molecular weight size-marker, this is a set of predetermined DNA fragment sizes from where unknown bands are compared to identify the approximate size and concentration of a DNA fragment after running gel electrophoresis.
DNA LADDER
Deoxynucleotide triphosphates (dNTPs), they represent the essential building blocks of nucleic acid molecules that are necessary components of PCR mixes during amplification. Four known types of dNTP are recognized, each with a unique DNA base: adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP).
dNTPs
disease which occurs in a limited geographic area.
endemic
3,8- Diamino-5-ethyl-6-phenylphenanthridinium, is a non-radioactive but carcinogenic DNA stain. Distinct red-orange fluorescence under UV light suggests the presence of ds-DNA.
ethidium bromide EtBr
agent causing the infection or disease
etiologic agent
this is a step in PCR when the temperature is increased and the new DNA strand is formed through the action of Taq polymerase. Temperature is usually set at 72°C.
extension
a property wherein a particle is able to pass through a membrane filter that will prevent the transmission of bacteria
filterable
property wherein a particle is able to pass through a membrane filter that will prevent the transmission of bacteria
filterable
cottony or wooly. Type of texture formed by large quantities of aerial hyphae usually arranged in a tangled manner.
floccose
aka 5’ primers, these are PCR primers that are complementary to the anti-sense strand of ds-DNA and therefore responsible for its amplification. It occurs at the 5’ end of the PCR product. Primarily, it anneals to the antisense strand or negative strand of the double-stranded DNA which runs from 3’ to 5’ direction. Antisense strand or the coding strand serves as the template strand for the synthesis of mRNA.
forward primer
presence of fungi in the blood. The most common agent of fungemia is Candida albicans
fungemia
capable of inhibiting the growth of fungi
fungicidal
capable of inhibiting the growth of fungi
fungicidal
refers to the range of different inherited
traits within a species
genetic diversity
Soil-seeking, earth-loving. A dermatophyte having soil as its primary reservoir.
geophilic
Soil-seeking, earth-loving. A dermatophyte having soil as its primary reservoir.
geophilic
an elongated or tube-like structure that emerges from a spore, with no constriction at the point of origin. The presence of germ tubes can be used to presumptively identify the presence of Candida albicans.
germ tube
an elongated or tube-like structure that emerges from a spore, with no constriction at the point of origin. The presence of germ tubes can be used to presumptively identify the presence of Candida albicans.
germ tube
biological agents that can potentially cause harm to humans and animals when exposed.
hazards
adherence of red blood cells on a virally infected cell culture.
hemadsorption
an acronym for High Efficiency Particulate Air) a mechanical filter that is capable of trapping and removing particles and microorganisms from the air.
HEPA
a member of the human Herpesviridae family that can cause oral and genital infections.
HSV
microscopic structures are clear or colorless.
hyaline
vegetative filament of a fungus.
hypha (pl. hyphae)
serologic test based on precipitation reaction to determine the presence of antibody to fungal antigen by double diffusion technique in gel medium.
immunodiffusion
refers to a personʼs complete set of chromosomes.
karyotype
simple serologic procedure wherein the antibody formed following a fungal disease is detected through the clumping of antigen coated particles.
latex agglutination
commercially available or laboratory prepared solutions used to standardize the approximate amount of bacteria or fungi in a liquid suspension
McFarland standard
a.k.a. candidiasis, a fungal infection caused by a yeast, Candida albicans.
moniliasis
fungal colonies composed of hyphae.
mould or mold
like a wall; multicellular, with both transverse and longitudinal septations.
muriform
colony with hyphae that do not produce conidia or spores
mycelia sterilia
mass of hyphae making up a fungus colony.
mycelium
pertaining to fungi
mycotic
clear zone of lysis due to successive infection of host cells by a virus
plaque
presence of two or more variant forms of a specific DNA sequence that happen among different individuals or populations. SNP or single nucleotide polymorphism is the most common type.
polymorphisms
general purpose basal medium made from potato infusion and dextrose used to isolate yeasts and molds
potato dextose agar
set of media inoculated from the specimen
primary culture
referring to the surface
obverse
place where the fungus is found in nature.
reservoir
the microbe is able to grow in the presence of a particular antimicrobial
resistant
the microbe is able to grow in the presence of a particular antimicrobial
RESISTANT
aka 3’ primers, these primers are complementary to the sense or the (+) strand of the ds-DNA and therefore responsible for its amplification. It occurs at the 3’ end of the PCR product. Primarily, it anneals to the sense strand or positive strand of the double-stranded DNA, which runs from 5’ to 3’ direction. The sense strand is complementary to the template strand and therefore, it is known as the anticoding strand.
REVERSE PRIMER
rootlike structures that anchors the fungus to the substrate and also utilized in the absorption
rhizoids
culture medium primarily used for fungal cultivation and isolation of dermatophytes
saboraud dextrose agar
an organism which requires organic material as a source of energy.
saprobe
the ability to detect all patients with a specific disease.
sensitivity
having cross walls or partitions.
septate
reproductive propagule produced internally by “free cellˮ formation, as in the ascomycete, i.e., complete spores formed all at once around the nuclei available or by “progressive cleavage” as in a sporangium.
spore
destruction of all life forms, including the bacterial spores.
sterilization
horizontal hypha from which hyphae, rhizoids or sporangiophore develop.
stolon
material on which the fungus is growing.
substrate
or also known as sensitive. The microbe is unable
to grow in the presence of a particular antimicrobial.
susceptible
thermostable DNA polymerase used during PCR amplification. It can withstand high temperatures without being denatured. It is extremely heat resistant with a half-life of 40 minutes at 95°C. It is derived from a heat tolerant bacterium, Thermus aquaticus. It is also abbreviated as Taq pol.
taq polymerase
used to preserve the integrity of the specimen from the time of collection up to the laboratory processing
transport medium
equipment used to visualize target DNAs and proteins by emitting high levels of ultraviolet rays through the viewing surface
UV transilluminator
human herpes virus that can cause the highly contagious chickenpox.
VZV
capable of inactivating or destroy viruses
virucidal
predominantly a unicellular fungus, usually round or
ovoid, that reproduces by budding.
yeast
culture medium that is useful for yeast identification made from peptone and yeast extract
yeast fermentation broth -
culture medium that is useful for yeast identification made from peptone and yeast extract
yeast fermentation broth -
a circular area surrounding the antibiotic disk showing no visible inoculum
zone of inhibition
a circular area surrounding the antibiotic disk showing no visible inoculum
zone of inhibition