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MYCOVIRO > BACTE UNIT 1 > Flashcards

BACTE UNIT 1 Flashcards

1
Q

recovery of aerobic organisms

A

SWABS

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2
Q

for Bacterial Culture / Good for fastidious bacteria

A

dacron, calcium alginate ,rayon

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3
Q

viral culture

A

cotton and dacron

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4
Q

flexible swab

A

nasopharyngeal swab

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5
Q

plastic or wooden material

A

shaft

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6
Q

prior to culture and sputum examination, pre-examone the submitted sputum

A

gram stain bartlett’s classification

> 25 leukocytes
<10 epithelial cells

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7
Q

catheter tips, CSF for viruses, outer ear swab, unpreserved feces and urine, feces (for isolation of Clostridioides difficile toxin), sputum, swabs

A

REF TEMP 4

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8
Q

CSF for bacteria, abscess, lesion, wound, body fluids, inner ear,
preserved urine and stool, nasal specimens, and tissues

A

AMBIENT ROOM TEMP 22

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9
Q

CSF FOR MENINGITIS

A

body temp 37

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10
Q

serum for serology

long term storage

A

-20

-70

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11
Q

Usual incubation time for most bacteria; AFB

A

18-24 hours at 37 degrees

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12
Q

Pseudomonas aeruginosa, Campylobacter jejuni

A

42 degrees

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13
Q

Listeria monocytogenes and Yersinia enterocolitica

A

4 degrees

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14
Q

Aerobic bacteria eg. P.aeruginosa

A

ambient air 21% oxygen

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15
Q

Anaerobic eg. Fusobacterium, Bacteroides, Clostridium

A

Gas-Pak, Brewer Jar, Glove Box method, Biobag System

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16
Q

Enterics

A

facultative anaerobes

17
Q

Capnophiles

A

510% carbon dioxide; Use of Candle Jar

18
Q

Microaerophiles eg. Campylobacter jejuni, Helicobacter pylori

A

reduced oxygen (5-6%)

19
Q

less than 2 hours

A

abscess, ear aspirates, conjunctival swab
specimens from female genital tract, prostatic swab, male urethral swab, lower respiratory tract
throat swab, straight catheter and unpreserved urine

20
Q

within 2 hours

A

blood, bone marrow aspirates

21
Q

within 24 hours

A

stool

22
Q

less than 15 minutes

A

body fluids
corneal scrapings
gastric aspirates

23
Q

suprapubic aspirate

A

immediately

24
Q

factors affecting microbial growth

A
  1. type of organism
  2. microbial load
  3. concentration of agents to be used
  4. presence of organic material
  5. nature of surface to be disinfected
  6. contact time
  7. temp and pH
  8. compatibility of disinfectants
25
Q

refers to the absence of microbes in an area or object.

A

asepsis

26
Q

refers to methods employed to prevent entry of organisms into the body as well into test tubes, flasks, or plates used in microbial cultivation.

A

aseptic technique

27
Q

involves chemical disinfection of living tissues (i.e. mucus membrane, skin) as well as in the treatment of wounds. The chemical agent employed is called antiseptic

A

ANTISEPSIS

28
Q

a condition in which microbial growth and multiplication is halted or inhibited. The microorganisms is not killed or destroyed in the process. Bacteriostatic methods include the use of refrigeration as well as dyes.

A

bacteriostasis

29
Q

microorganisms inadvertently introduced to specimens or bacterial culture.

A

contaminant

30
Q

the process of removal of pathogenic microorganisms so materials/items are safe to handle or dispose.

A

DECONTAMINATION

31
Q

removal of transient microorganisms from the skin using mechanical cleansing or antiseptics.

A

demerging

32
Q

process of destruction of pathogenic microorganisms except spores, prions and other microorganisms.

A

DISINFECTION

33
Q

destruction of all forms of life including bacterial spores.

A

STERILIZATION

34
Q

lowest temperature in which all bacteria in a liquid culture will be killed in 10 minutes

A

thermal death point

35
Q

minimal length of time in which all bacteria in a liquid suspension will be killed at a given temperature

A

thermal death time

36
Q

Factors influencing the degree of killing

A
  1. types of organisms
  2. number of organisms
    concentration or compatibility of disinfectant
  3. presence of organic material
  4. nature of surface to be disinfected
  5. contact time
  6. temperature
  7. pH
  8. biofilm
37
Q

unacceptable and rejected specimens

A
  1. request and specimen mismatch (wrong label)
  2. improper transport container
  3. QNS
  4. unpreserved specimens (>2hrs)
  5. fixed specimens
  6. improper collection
  7. sputum (bartlett’s classification)
38
Q

unacceptable and rejected specimens

A
  1. request and specimen mismatch (wrong label)
  2. improper transport container
  3. QNS
  4. unpreserved specimens (>2hrs)
  5. fixed specimens
  6. improper collection
  7. sputum (bartlett’s classification)

MYCOVIRO (4 decks)

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