BACTE UNIT 1.2

Question 1

Isolation of gram-positive and gram-negative anaerobes

Answer 1

Anaerobic phenylethyl alcohol agar

Question 2

Isolation of gram-negative anaerobes

Answer 2

Anaerobic kanamycin-vancomycin agar

Question 3

Isolation of Bordetella pertussis

Answer 3

Bordet-Gengou media
regan- lowe agar

Question 4

Enrichment used to isolate
Legionella

Answer 4

Buffered charcoal yeast extract

Question 5

Isolation of Campylobacter; Brucella agar and sheep blood with antibiotics

Answer 5

Campy blood agar

Question 6

Enriched, nonselective medium to isolate fastidious organisms, such as Neisseria and Haemophilus

Answer 6

chocolate agar

Question 7

Isolation of Yersinia species

Answer 7

Cefsulodin-Irgasan-nov obiocin agar

Question 8

Isolation of gram-positive bacteria

Answer 8

Colistin-nalidixic acid agar

Question 9

Differential: isolation of gram-negative bacilli and differentiation of lactose fermenters from non-lactose fermenters

Answer 9

eosin-methylene blue agar and mac conkey agar

Question 10

Enrichment broth to enhance recovery of stool pathogens and inhibit normal flora coliforms

Answer 10

gram-negative broth
selenite
tetrathionate

Question 11

Selective: isolation of stool pathogens through inhibition of normal flora coliforms

Answer 11

Hektoen enteric agar
xylose-lysine
salmonella-shigella

Question 12

Selective: isolation of stool pathogens through inhibition of normal flora coliforms

Answer 12

hektoen enteric agar

Question 13

Primary isolation medium for Mycobacterium

Answer 13

Löwenstein-Jensen medium
Middlebrook medium
petrangani’s medium

Question 14

Isolation of Neisseria gonorrhoeae

Answer 14

Martin-Lewis medium and modified thayer- martin agar

Question 15

Selective: isolation of stool pathogens through inhibition of normal flora
coliforms

Answer 15

Salmonella-Shigella agar

Xylose-lysine- deoxycolate agar

hektoen enteric agar

Question 16

Isolation and differentiation of Vibrio

Answer 16

Thiosulfate-citrate-bile salts-sucrose agar

Question 17

All-purpose isolation media; subculture from primary plate

Answer 17

trypticase soy agar

Question 18

Enrichment broth to enhance recovery of stool pathogens and inhibit normal flora coliform

Answer 18

gram-negative broth
Selenite broth
tetrathionate broth

Question 19

Enriched broth that permits growth at various oxygen levels

Answer 19

thioglycolate broth