Germ Plasm and Germ Cells Flashcards

1
Q

Germ plasm generally has a visible different appearance due to

A

P granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

2 basic components Germ plasm contains:

A

mitochondria, and RNA helicases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What kind of cells in general, do the germ cells Z2 and Z3 come from in C elegans?

A

They are derived from the posterior cells P1-4.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are two components that track with germ cells in C Elegans?

A

PIE - I and p granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What gene is required for putting germ plasm in the proper posterior cells?

A

par genes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

In order to remain germ cells, transcription must be repressed by ____ to not differentiate

A

PIE-I

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the name of germ cell precursors in Drosophila?

A

pole cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How can pole plasm be proved as a localized determinant?

A

take pole plasm from the posterior of one fly, inject into the anterior of a second to induce pole cells, then take those cells and place them in a third fly anterior near the gonads and develop. Look for cells of the second genotype in the final fly.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How to test if oskar mRNA is sufficient to recruit germ plasm [create pole cells]

A

bicoid mRNA is normally at the anterior. Add bicoid localization signal to an oskar gene and see pole cells develop at the anterior

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How are primordial germ cells directed into the genital ridge?

A

They are guided via chemotaxis by SDF-1.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the name of the receptor that senses SDF-1?

A

The receptor is CXCR4.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What happens when you add too much SDF1 into an embryo? What analogy did Dr. Hardin make?

A

You get germ cells going everywhere, and not localizing. Like the Hardin wife’s perfume example.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly