Genetics Translation Flashcards
All amino acids have a […] an […], and a […]. An enzyme of the ribosome catalyzes formation of a […] between the carboxyl group of one amino acid and the amino group of the next
The […] of each amino acid is distinct; some are not charged, some are polar, and some are […].
central carbon; an amino group; carboxyl group; peptide bond; R-group; electrically charged
Polypeptides are strings of […] that are assembled by ribosomes. Ribosomes are “machines” that contain multiple […] and proteins. Ribosomes translate mRNA in the […] direction, reading each triplet codon and assembling the amino acids in the order specified by the codons. Specific tRNAs bound to amino acids (charged tRNAs) […] the appropriate amino acid into the ribosome
amino acids; ribosomal RNAs; 5′-to-3′; deliver
Ribosomes in bacteria, archaea, and eukaryotes perform three tasks: Bind [..] and identify the start codon, where translation begins, Facilitate complementary base pairing of […] and the corresponding tRNA anticodons. Catalyze formation of peptide bonds between amino acids on the growing […].
mRNA; mRNA codons; polypeptide chain
Ribosome composition—number and sequence of […] molecules and number and type of proteins—differs between bacteria, archaea, and eukaryotes. Ribosomes are composed of two subunits, the […] ribosomal subunit and the [..] ribosomal subunit. Ribosomal subunit size is measured in […] units (S), a property based on size, shape, and hydration state
rRNA; large; small; Svedberg
The […] site (P site) holds the tRNA to which the polypeptide is attached. The […] site (A site) binds a new tRNA molecule containing an amino acid to be added to the growing polypeptide chain. The […] site (E site) provides an avenue for exit of the tRNA after its amino acid has been added to the chain. The large subunit contains a channel from which the polypeptide chain […].
peptidyl; aminoacyl; emerges
Translation can be divided into three phases: […], […], […]. The phases are similar in bacteria and eukaryotes, though there are several […]. The differences lie largely in how the […] is identified when translation is initiated
Initiation; Elongation; Termination; differences; start codon
Initiation begins when the [….] binds near the 5′ end of the mRNA and identifies the start codon. The initiator tRNA, carrying the first […] of the polypeptide, binds to the start codon
Finally, the large subunit joins the small subunit to form the intact ribosome and […] begins.
ribosomal subunit; amino acid; translation begins
[…] factor proteins help control ribosome formation and binding of the initiator tRNA. GTP provides the energy for […]. tRNAs used during translation that carry an amino acid are called […] tRNAs, whereas tRNAs without amino acids attached are uncharged.
Initiation; initiation; charged
The preinitiation complex forms when the 16S rRNA and the […] sequence on the mRNA base pair. The Shine–Dalgarno sequence is a […] sequence of about six nucleotides located three to nine nucleotides upstream of the start codon. A complementary pyrimidine-rich sequence is found near the 3′ end of the 16S […]. For most of initiation, the 30S subunit is affiliated with an […], IF3, which prevents the 30S subunit from binding the 50S subunit
Shine–Dalgarno; purine-rich; rRNA; initiation factor
The initiator tRNA binds to the start codon where the […] will be once the ribosome is fully assembled. Initiator tRNA carries a modified […], N-formylmethionine (f Met); the charged. initiator t R N A is called […]. IF2 and GTP help facilitate binding of […]. IF1 joins the […]. Together these form the […] initiation complex.
P site; amino acid; tRNA fmet; complex; 30S
In the last stage of initiation, the 50S subunit joins the 30S subunit to form the intact […] . The union of the two subunits is driven by […] of GTP to GDP. The dissociation of IF1, IF2, and IF3 accompanies the joining of the subunits to create the 70S […] complex
ribosome; hydrolysis; initiation
The correct start codon (A U G) can be located because it is embedded in a […]: 5’-ACCAUGG-3’. This sequence is called the […] sequence, after its discoverer, Marilyn Kozak. Location of the start codon leads to recruitment of the 60S subunit to the complex, using energy from […], and the dissociation of the e I F proteins.
consensus sequence; Kozak; GTP hydrolysis
Elongation begins with recruitment of […] (E F) proteins and uses energy of GTP hydrolysis to: Recruit charged tRNAs to the […]. Form […] between sequential amino acids. Translocate the ribosome in the […] direction along the mRNA.
elongation factor; A site; peptide bonds; 3′
Several different EFs and other ribosomal proteins carry out […] in a series of steps. EF-Tu and GTP carry charged tRNAs to the […]; the tRNA with the correct […] sequence pairs with the codon. When a tRNA pairs with the […] codon, hydrolysis of GTP releases EF-Tu-GDP from tRNA. […] catalyzes peptide bond formation between amino acids at the P and A sites, elongating the polypeptide and transferring it to the tRNA at the […].
elongation; anticodon; mRNA; Peptidyl transferase; A site
The tRNA from the P site then exits through the [..]. EF-G translocates the ribosome, using GTP hydrolysis, moving it […] nucleotides toward the 3′ end of the m R N A. This moves the t R N A at the [..] to the [..] and opens the A site for the next charged t R N A carrying the correct anticodon.
E site; three; A site; P site
Distinct elongation factors carry out elongation in […] and archaea and the steps are similar to those of bacteria. Based on sequence, archaeal and eukaryotic […] are more alike than archaeal and bacterial E Fs. This sequence comparison reinforces the conclusion that eukaryotes and archaea are more […] to one another than either is to bacteria.
eukaryotes; EF homologs; closely related
The elongation cycle continues until one of the three stop codons (U A A, U A G, U G A) enters the […] of the ribosome. All organisms use […] to bind a stop codon in the A site. The polypeptide bound to the t R N A at the […] is then released while the RF is ejected and the ribosomal subunits separate.
A site; release factors; P site
In bacteria, the release factor […] recognizes UAG and UAA and […] recognizes UAA and UGA, while […] recycles RF1. Eukaryotic and archaeal termination is accomplished by a single release factor […] in eukaryotes and a RF1 in archaea, each of which recognizes all three stop codons
Eukaryotes have a […] release factor that recycles e R F 1
RF1; RF2; RF3; eRF1; second
Cell biologists estimate that each bacterial cell contains about […] ribosomes, collectively accounting for 25% of the mass of the cell
[…] micrographs reveal structures called polyribosomes, containing groups of ribosomes all actively translating the same […].
20,000; Electron; m R N A
Each polypeptide-producing gene in eukaryotes produces […] mRNA, an RNA that directs synthesis of a single kind of polypeptide. Groups of bacterial and archaeal genes, called […], often share a single promoter and produce polycistronic m R N As that lead to synthesis of several different proteins
The genes of operons function in the same […] pathway and are regulated as a unit.
monocistronic; operons; metabolic
[…] m R N As contain multiple polypeptide-producing segments, each with a start and stop codon. In bacterial and all but leaderless archaeal mRNAs, most translation-initiating regions contain a […] site. An […] spacer sequence that is not translated separates the segments. When the spacer sequences are short (a few nucleotides long), intact ribosomes may proceed to the next start codon after finishing […] of the previous segment
Polycistronic; Shine–Dalgarno; intercistronic; translation
The term […] describes the correspondence between m R N A codon sequences and the amino acid sequences of the resulting polypeptides
Transfer R N As are […] molecules that interpret and then act on information carried in m R N A
They have [….] complementary to m R N A codons
“genetic code”; adaptor; anticodon sequences
Groups of three consecutive nucleotides (codons) in an m R N A each correspond to […] amino acid
The genetic code contains […] different codons; with only […] common amino acids, this leads to redundancy—some amino acids are specified by more than […] codon
one; 64; 20; one
The […] , with 64 combinations, provides enough variety to code 20 amino acids. 61 codons specify […], and 3 are stop codons. All amino acids except methionine and tryptophan are specified by at least two codons, called […] codons
triplet genetic code; amino acids; synonymous