General MicroStuff

Question 1

Indian Ink Stain and Mucicarmine

Answer 1

Cryptococcus Neoformans “sneaking around india”

Question 2

Silver Stain bugs (3)?

Answer 2

Fungi (eg Pneumocystis), Legionella, H. Pylori.

“Legionaires wear silver armor”

Question 3

Chocolate Agar with V and X (what are V/X?)

Answer 3

H. INfluenzae (V=NAD+) (X=Hematin)

Question 4

Thayer-Martin Media

Answer 4

Neisseria G/M. “TMN- teenage mutant ninja turtles”

Question 5

Bordet-Gengou Agar

Answer 5

Bordetella Pertussis (“Bordet for Boretella”)

Question 6

Tellurite Agar, Loffler Medium

Answer 6

Clostridium Diphtheriae “tell you right, you’re a cougher w/ diptheriae”

Question 7

Lowenstein-Jesnen Agar

Answer 7

Mycoplasma Tuberculosis (“L J M ~ J-k-L-M”)

Question 8

Eaton agar with Cholesterol

Answer 8

Myco Pneumoniae

Question 9

Pink on MacConkey

Answer 9

Lactose-Fermenting Enterics (producing acid=pink)

Question 10

Legionella

Answer 10

Charcoal Yeast with cyestine and Iron

Question 11

Sabouraud Agar

Answer 11

Fungi “sabs a fun guy!”

Question 12

Obligate Aerobes

Answer 12

Nagging Pests Must Breathe:

Nocardia, Pseudomonas, MycoBacterium

Question 13

Obligate Anaerobes

Answer 13

Anaerobes C-ant B-reathe A-ir (lack catalse and/or superoxide dysmutase = oxidative damage):
Clostridium, Baceteroides, Actinomyces

Question 14

Obligate intracellular (why?)

Answer 14

Can’t make own ATP; Ricekettsia and Chlamydia

Question 15

Facultative Intracellular

Answer 15

Some Nasty Bugs May Live FaculatativeLY:

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis

Question 16

Encapsulated Bacteria

Answer 16

SHiNE SKiS:

Strep Pneumo, H. Influ, Neisseria, E coli, Salmonella, Kleb, group B Strep

Question 17

Catalase Positive Organisms (what disease are they prominent in?)

Answer 17

PLACESS for you cat(alase).
Pseudomonas, Listeria, Aspergillus, Candida, E.coli, S aureus, Serratia
Chronic Granulomatous Disease (lacking NADPH Oxidase)

Question 18

Urease-Positive Bugs

Answer 18

PUNCH KSS:

Proteus, Ureaplasma, Nocardia, Cryptococcus, H. Pylori, Klebseilla, S. Epi, S. Saprophyticus

Question 19

Protein A (mechanism and organism)

Answer 19

binds Fc region of IgG, preventing phagocytosis of S. Aureus.
A-ureus A-voids A-ttack with protein A.

Question 20

IgA protease (mechanism and organisms)

Answer 20

Cleaves IgA. (SHiN): Strep Pneumon, H. Influe, Neisseria.

Question 21

M Protein

Answer 21

Prevents phagocytosis of Group A Strep (eg pyrogens).

M protein=M-olecular M-imicry M-itral Valve (regurg/stenosis)

Question 22

Klebsiella

Answer 22

4 A’s:

Aspiration pneumonia, Abscesses in lung/liver (double L in kleb), Alocholics, and diAbetics.

Question 23

Rash on palms and soles

Answer 23

you drive “CARS” using your palms and soles:

“Coxsackie A, Rickettsia (rocky mnt–really east coast), Syphyllis (secondary)

Question 24

Reiters syndrome

Answer 24

Reactive arthritis post GI (shigella, salmonella, yersinia, camplyobacter) or chlamydial infections

“Can’t see (conjnctivitis), can’t pee (urethritis), can’t bend my knee (arthritis)”

Question 25

Lactose Fermenters

Answer 25

SEEK: Pink on MacConkey.

Serratia, Enterobacter, E. Coli, Klebsiella

Question 26

Ear infection? Bacterial causes and difference between the them?

Answer 26

• H. Flu: Otitis MEDIA: acute in kids/adults. Shows bulging of the tympanic membrane.
• Pseudomonas: Malignant otitis EXTERNA (outside of tympanic membrane). Elderly diabetics. Can progress to osteomyletis and cranial nerve deficits.

Question 27

Quellung reaction?

Answer 27

Used to identify capsular bacteria–they will swell upon addition of anti-capsular reagant antibodies (“Quellung causes capsular swelling”)