Gene Expression Flashcards

1
Q

Name the 2 stages involved in gene
expression of DNA sequences

A

Transcription and Translation

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2
Q

How many strands make up RNA?

A

1

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3
Q

Describe the complementary base
pairing in RNA

A

Guanine pairs with Cytosine
Adenine pairs with Uracil

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4
Q

Name the components that make up
an RNA nucleotide

A

Ribose sugar, phosphate and a base

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5
Q

Name the 3 types of RNA

A

mRNA, tRNA and rRNA

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6
Q

The name given to three bases on a
DNA molecule

A

Triplet

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7
Q

The function of mRNA

A

Carries a copy of DNA from the
nucleus to the ribosome

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8
Q

Which molecule carries a
specific amino acid to the ribosome?

A

tRNA

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9
Q

The name for a triplet of bases on
an mRNA molecule that codes for a
specific amino acid

A

codon

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10
Q

What causes tRNA to fold?

A

complimentary base pairing

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11
Q

Name the 2 areas on a tRNA
molecule

A

Anticodon and an attachment site
for an amino acid

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12
Q

Name for a triplet of bases on
tRNA

A

anticodon

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13
Q

Name the 2 structures that form
the ribosome

A

Ribosomal RNA (rRNA) and protein

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14
Q

Describe the function of RNA
polymerase

A

Unwinds DNA double helix and
breaks hydrogen bonds between
base pairs to separate strands

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15
Q

What forms when complementary
base pairing occurs between free
RNA nucleotides and the open
template strand?

A

Primary Transcript

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16
Q

The name for non-coding regions on a
primary transcript of mRNA

17
Q

The name for coding regions on a
primary transcript of mRNA

18
Q

What process leads to the
formation of a mature transcript?

A

RNA splicing

19
Q

Describe what occurs during RNA
splicing

A

Introns are removed and exons
are joined together

20
Q

Where does translation begin and
end?

A

at start and stop codons

21
Q

How do anticodons on tRNA bond to
codons on mRNA?

A

complimentary base pairing

22
Q

The purpose of translation

A

To convert the DNA genetic code
into a sequence of amino acids
forming a polypeptide

23
Q

The bonds that join amino acids
together

24
Q

Structure formed when amino acids
are joined by peptide bonds

A

polypeptide

25
What happens to the tRNA as a polypeptide is formed?
It leaves the ribosome to pick up another amino acid
26
How can different proteins be expressed from one gene?
Alternative RNA splicing
27
How can different mature mRNA transcripts be produced from the same primary transcript?
Different exons are retained
28
How is the 3D shape of a protein formed?
Polypeptide chains formed during translation fold
29
How are proteins held together?
Hydrogen bonds and individual interactions between individual amino acids
30
What does the shape of a protein determine?
the function
31
How is phenotype determined?
By proteins produced through gene expression
32
Requirements of PCR
Original DNA sample Primers DNA polymerase nucleotides
33
Requirements of DNA replication
Original DNA template nucleotides enzymes (DNA Polymerase and DNA Ligase) ATP Primers
34
What is Transcription?
when mRNA is converted from DNA creating a primary transcript in the nucleus
35
What is translation
When mRNA information is converted into a polypeptide at the ribosomes