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Biology 207 > Gel Electrophoresis > Flashcards

Gel Electrophoresis Flashcards

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1
Q

Electrophoresis

A

The separation of charged molecules using an electric field

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2
Q

What type of gel do we use in the lab?

A

Agarose gel

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3
Q

What is in the DNA mix?

A

DNA molecules
Glycerol to add weight
Loading/tracking dye

Dropped into a well

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4
Q

What is the result of electrophoresis?

A

Linear pieces of dsDNA move according to length

Pieces of DNA the same length form a band in their lane

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5
Q

What are molecular weight markers?

A

dsDNA fragments of known sizes and amounts

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6
Q

How do we determine the length of DNA?

A
  1. Run a gel with unknown DNA in one lane and molecular weight markers in the second lane
  2. Take a photograph
  3. Measure distances each band travelled from well
  4. Print a sheet of semi-log paper
  5. Plot the distance of the molecular weight marker bands
  6. Estimate the size of the unknown band
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Biology 207 (40 decks)

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