Cloning a Gene Flashcards
1
Q
How is a recombinant chromosome made?
A
Made in vivo by crossovers during meiosis
2
Q
How is recombinant DNA made?
A
Made in vitro by geneticists
3
Q
What is gene cloning?
A
Using bacterial cells to make identical copies of a specific gene
4
Q
What are the tools needed to clone genes?
A
Insert DNA Vector DNA Ligate Recombinant DNA Bacteria cell
5
Q
What are cells with R6K resistant to?
A
Penicillin, ampicillin
6
Q
What can cells with ColE1 do?
A
Kill cells that don’t have it
7
Q
What does T4 DNA ligase make?
A
Covalent bonds
8
Q
What are the properties of E. coli DH5alpha?
A
Mutations in genes that make restriction enzymes
9
Q
What does it mean when a cell is competent?
A
Temporary holes in the cell wall
10
Q
The steps to cloning a gene
A
- Prepare the insert DNA - optional is to gel purify the insert DNA
- Prepare the vector DNA
- Mix DNAs and add T4 DNA ligase
- Transform competent cells
- Plate cells
- Remove cells from the colony
