Flow Cytometric Analysis in Hematologic Disorders

Question 1

1. What is the most common clinical application of flow
   cytometry?
   a. Diagnosis of platelet disorders
   b. Detection of fetomaternal hemorrhage
   c. Diagnosis of leukemias and lymphomas
   d. Differentiation of anemias

Answer 1

c. Diagnosis of leukemias and lymphomas

Question 2

2. Which of the following is true of CD45 antigen?
   a. It is present on every cell subpopulation in the bone
   marrow.
   b. It is expressed on all hematopoietic cells, with the exception of megakaryocytes and late erythroid precursors.
   c. It is not measured routinely in flow cytometry.
   d. It may be present on nonhematopoietic cells.

Answer 2

b. It is expressed on all hematopoietic cells, with the exception of megakaryocytes and late erythroid precursors.

Question 3

3. Erythroid precursors are characterized by the expression of:
   a. CD71
   b. CD20
   c. CD61
   d. CD3

Answer 3

a. CD71

Question 4

4. In Figure 32-2A, the cell population colored in aqua
   represents:
   a. Monocytes
   b. Nonhematopoietic cells
   c. Granulocytes
   d. Lymphocytes

Answer 4

d. Lymphocytes

Question 5

5. Antigens expressed by B-LL include:
   a. CD3, CD4, and CD8
   b. CD19, CD34, and CD10
   c. There are no antigens specific for B-LL.
   d. Myeloperoxidase

Answer 5

b. CD19, CD34, and CD10

Question 6

6. Which of the following is true of flow cytometric gating?
   a. It is best defined as selection of a target population for
   flow cytometric analysis.
   b. It can be done only at the time of data acquisition.
   c. It can be done only at the time of final analysis and
   interpretation of flow cytometric data.
   d. It is accomplished by adjusting flow rate.

Answer 6

a. It is best defined as selection of a target population for
flow cytometric analysis.

Question 7

7. Collection of ungated events:
   a. Facilitates comprehensive analysis of all cells
   b. Does not help in detection of unexpected abnormal
   populations
   c. Allows the collection of data on a large number of rare cells
   d. Is used for leukemia diagnosis only

Answer 7

a. Facilitates comprehensive analysis of all cells

Question 8

8. Mycosis fungoides is characterized by:
   a. Loss of certain antigens compared with the normal T cell
   population
   b. Polyclonal T cell receptor
   c. Immunophenotype indistinguishable from that of normal T cells
   d. Expression of CD3 and CD8 antigens

Answer 8

a. Loss of certain antigens compared with the normal T cell
population

Question 9

9. Mature granulocytes show the expression of:
   a. CD15, CD33, and CD34
   b. CD15, CD33, and CD41
   c. CD15, CD33, and CD13
   d. CD15, CD33, and CD7

Answer 9

c. CD15, CD33, and CD13

Question 10

10. During the initial evaluation of flow cytometric data, cell
    size, cytoplasmic complexity, and expression of CD45
    antigen are used to define cell subpopulations. Which of
    the following parameters defines cytoplasmic complexity/
    granularity?
    a. SS
    b. FS
    c. CD45
    d. HLA-DR

Answer 10

a. SS

Question 11

11. The most important feature of the mature neoplastic B cell
    population is:
    a. The presence of a specific immunophenotype with
    expression of CD19 antigen
    b. A clonal light chain expression (i.e., exclusively k- or
    l-positive population)
    c. A clonal T cell receptor expression
    d. Aberrant expression of CD5 antigen on CD191 cell

Answer 11

b. A clonal light chain expression (i.e., exclusively k- or
l-positive population)