Final (SLE AND MS) Flashcards

1
Q

SLE is different than RA because its a ___________ autoimmune disease

A

systemic

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2
Q

SLE affects what population more?

A

women
ethic

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3
Q

What are 5 common themes of SLE?

A
  1. auto-reactive B cells
  2. anti-nucleic acid auto-Ab
  3. pDCs
  4. TLR7/9
  5. IFN-a
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4
Q

where are auto-reactive B cells made in SLE?

A

extra-follicular B cell activation

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5
Q

How are anti-nucleic acid auto-Ab made in SLE?

A

cell damage from mutations exposes nucleic acids

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6
Q

How are pDCs involved in SLE?

A

DNA/RNA released from damaged cells is recognized by pDCs TLR7/9 activating them

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7
Q

How is TLR-7/9 involved in SLE?

A

recognizes released DNA/RNA from cell damage

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8
Q

How is IFN-a involved in SLE?

A

produced by activated pDCs when their TLRs recognize RNA/DNA from damaged cells
**all nucleated cells have IFN-a receptors

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9
Q

What is the SLE loop?

A

production IFN-a from activated pDCs activates formation of auto-reactive B cells and anti-nucleic acid auto-Abs

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10
Q

What induces the formation of auto-reactive B cells and anti-nucleic acid auto-Abs in SLE?

A

secretion of IFN-a

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11
Q

What is the entry criteria for SLE?

A

positive ANA

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12
Q

What is ANA?

A

antibodies against nuclear material

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13
Q

What are 3 ANAs in SLE?

A
  1. anti-dsDNA
  2. anti-histamine
  3. anti-Smith (RNA)
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14
Q

What is the clinical criteria for SLE?

A

fever
nephritis
seizures

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15
Q

What is the SLE flare?

A

waxing and waning of SLE symptoms

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16
Q

During the SLE flare what is elevated?

A

anti-DNA Ab
TLR-9 activated pDCs
IFN-a
* increased risk of nephritis and cardiac events

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17
Q

What is the immunological criteria for SLE?

A

decreased C3 and C4
increased anti-dsDNA & anti-Smith Ab

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18
Q

What does it mean when C3 and C4 is low?

A

less complement activation = less tagging and clearance = more chance for B cells to encounter DNA/RNA and produce auto-Ab

19
Q

What is the best immunological marker for SLE?

A

anti-Smith Ab

20
Q

What are 3 possible sources of DNA/RNA that activates pDCs in SLE?

A
  1. cell damage + LL37 + TLR-7 + pDCs
  2. RNA/DNA Ab internalized by pDCs
  3. loss of function of DNASE1L3
21
Q

What is the regular function of DNASE1L3?

A

breaks down DNA/RNA from apoptosed cells

22
Q

How does the loss of function of DNASE1L3 play a role in SLE?

A

large DNA/RNA from apoptosed cells cannot be broken down resulting in activation of pDCs (TLR-9/7) = IFN-a secretion

23
Q

What gene makes SLE a monogenic disease?

A

DNASE1L3

24
Q

Belimumab is an anti-BAFF treatment for SLE. How does it work?

A

prevents auto-reactive B cells from leaving the spleen
(BAFF is a B cell survivial signal)

25
Q

Anifrolumab is a ant-IFNRA treatment for SLE. How does it work?

A

prevents mDCs from signaling to B cells to create auto-reactive B cells

26
Q

Vocolsporin is a anti-Cal & synaptopin protecting drug. How does it work?

A

prevents transcription of NFAT = decreased T cells and cytokine production
protects podocytes (kidney filatration cells) *stabalizes cytoskeleton

27
Q

What is multiple sclerosis?

A

neuroinflammatory disease caused by immune complexes infiltrating the blood brain barrier

28
Q

What is the clinical manifestation of MS?

A

lesions on CNS caused by immune complex mediated inflammation

29
Q

What cells cause damage to the myelin sheath in MS?

A

CD8+ T cells and Ab attach to attract other immune cells

30
Q

What are the 3 types of MS?

A
  1. relapsing-remitting
  2. secondary progressive
  3. primary progressive
31
Q

What is relapsing-remitting MS?

A

waxing and waning of symptoms
* increased inflammation and demylenation when waxing

32
Q

Where are lesions on brains seen and by what imaging?

A

NMR
white matter

33
Q

What is secondary progressive MS?

A

waxing and waning but overtime recovery is eventually not possible

34
Q

What is primary progressive MS?

A

never experience remission (directly to progressive disease)

35
Q

What does it mean that the brain is immune privileged?

A

there is typically not immune responses there

36
Q

Where does immune response initiate in MS?

A

outside of CNS CD8+ T cells are activated then cross BBB

37
Q

Natalizumab is an anti-alpha4-beta1 integrin binding drug for MS. How does it work?

A

blocks CD8+ T cells from crossing the BBB = improved RR MS

38
Q

What are B cell’s role in MS?

A

make auto-Ab that bind to myelin to make macrophages eat mylein

39
Q

Ocrelizumab is an anti-CD20 therapy. How does it work for MS?

A

absence of CD20 induces B cell death = less auto-B cells and Ab

40
Q

S1P Modulators are drugs for MS. How?

A

prevents auto-reactive T cells from leaving the thymus

41
Q

Anti-IFN-B is a drug for MS. How?

A

limits inflammatory response

42
Q

What is EBV role in MS?

A

creates auto-reactive B cells against myelin which then presents to T cells making then auto-CD8+ T cells against myelin

43
Q

What is EBVs role in MS via molecular mimicry?

A

EBV viral protein sequences mimic human myelin proteins and thereby induce autoimmunity against myelin and CNS antigens

44
Q

What is a unique way auto-Ab are made in SLE?

A

B cell activation occurs outside of SLT (thanks to increased pDCs and IFN)