Affinity

Question 1

What are 4 types of non-covalent interactions?

Answer 1

hydrogen bonds
ionic bonds
van der Waal interactions
hydrophobic interactions

Question 2

What unit is the Kd measured in?

Answer 2

M

Question 3

What unit is the Ka measured in?

Answer 3

M-1

Question 4

Kd and Ka are _________ of each other

Answer 4

inverses

Question 5

What is the Kd?

Answer 5

concentration of L when 50% of RL complex is formed

Question 6

When 10% of RL is formed = ____xKd

Answer 6

0.1 x Kd

Question 7

When 50% of RL is formed = ____xKd

Answer 7

1 x Kd

Question 8

When 90% of RL is formed = ____xKd

Answer 8

10 x Kd

Question 9

When 100% of RL is formed = ____xKd

Answer 9

100 x Kd

Question 10

If Ka is a constant, why does binding increase with increasing concentration?

Answer 10

increasing concentration increases the probability of an interaction between R and L

Question 11

The smaller the Kd the better or worse the affinity?

Answer 11

better

Question 12

What is avidity?

Answer 12

enhanced affinity interaction due to multiple binding interactions

Question 13

What 2 things are required for avidity?

Answer 13

1. R of L attached to surface
2. requires other complement to have multiple binding sites

Question 14

What non-covalent interaction is important for specificity?

Answer 14

van der Waal