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BioChem Lab > Exp. 5 & 6_Post Lab > Flashcards

Exp. 5 & 6_Post Lab Flashcards

1
Q

stores the genetic information of an organism

A

DNA

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2
Q

transmits that information from one generation to another.

A

DNA

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3
Q

translates the genetic information contained in DNA into proteins needed for all cellular function

A

RNA

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4
Q

Responsible for protein synthesis

A

Nucleic Acid

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5
Q

A nucleic acid is an

A

unbranched polymer

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6
Q

monomer units of nucleic acids are

A

nucleotides

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7
Q

A___________ is an unbranched polymer in which the monomer units are nucleotides.

A

nucleic acid

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8
Q

T or F| ALL nucleic acid molecules are unbranched polymers.

A

Trueee!!!

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9
Q

A nucleotide is composed of:

A

Nitrogen-containing bases (amines)
Sugar
Phosphate

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10
Q

T or F| ATP is an example of a nucleotide

A

TRUEEE

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11
Q

What is ATP composed of?

A

Adenine
3 phosphate
Ribose

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12
Q

Nitrogen containing bases

A

Purines
Pyramidines

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13
Q

2 ringed nitrogen group

A

Purines

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14
Q

1 ringed nitrogen group

A

Pyrimidines

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15
Q

Purines of DNA and RNA

A

Adenine
Guanine

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16
Q

Pyrimidines of DNA

A

Cytosine
Thymine

17
Q

Pyrimidines of RNA

A

Cytosine
Uracil

18
Q

Sugar of RNA

A

Ribose

19
Q

Sugar of DNA

A

Deoxyribose

20
Q
  • crush cell wall
A

Blender

21
Q

Homogenizing solution

A

Salt and Dishwashing liquid

22
Q
  • removes the lipids and push DNA to the salt solution
A

dishwashing liquid

23
Q
  • increases separation of DNA from hydrophobic layer; aggregates DNA
A

sodium chloride

24
Q

– promotes aggregation of DNA and wash the DNA from the salt

A

cold ethanol

25
Q

Test for the deoxyribose in DNA

A

Dische Diphenylamine Test

26
Q

Dische Test: Reagents

A

diphenylamine

Sulfuric acid

27
Q

Dische test positive results

A

light blue to blue depending on the amount of DNA

28
Q

Dische Test: GLUCOSE

A

Grayish Brown

29
Q

Dische Test: RIBOSE

A

Greenish brown

30
Q

Dische Test: DEOXYRIBOSE

A

Bright Blue

31
Q

Dische Test: EXTRACTED DNA

A

Brownish Blue

32
Q

Hydrolysis of DNA molecule

A

Acid Hydrolysis

33
Q

breakdown of glycosidic bonds only if pH > 3

A

Depurination

34
Q

Depurination
breakdown of glycosidic bonds only if pH is

A

pH > 3

More than 3

35
Q

Products of Depurination

A

purine base
deoxyribose with phosphate group

36
Q

breakdown of glycosidic bond with phosphodiester bond if pH < 2

A

Total breakdown

37
Q

Total breakdown
breakdown of glycosidic bond with phosphodiester bond if pH is

A

pH < 2

Less than 2

38
Q

Extremely low pH_____ DNA completely

A

hydrolyses

39
Q

Products of total breakdown

A

phosphate group

purine or pyrimidine base

deoxyribose

BioChem Lab (17 decks)

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