Exp. 5 & 6_Post Lab Flashcards
stores the genetic information of an organism
DNA
transmits that information from one generation to another.
DNA
translates the genetic information contained in DNA into proteins needed for all cellular function
RNA
Responsible for protein synthesis
Nucleic Acid
A nucleic acid is an
unbranched polymer
monomer units of nucleic acids are
nucleotides
A___________ is an unbranched polymer in which the monomer units are nucleotides.
nucleic acid
T or F| ALL nucleic acid molecules are unbranched polymers.
Trueee!!!
A nucleotide is composed of:
Nitrogen-containing bases (amines)
Sugar
Phosphate
T or F| ATP is an example of a nucleotide
TRUEEE
What is ATP composed of?
Adenine
3 phosphate
Ribose
Nitrogen containing bases
Purines
Pyramidines
2 ringed nitrogen group
Purines
1 ringed nitrogen group
Pyrimidines
Purines of DNA and RNA
Adenine
Guanine
Pyrimidines of DNA
Cytosine
Thymine
Pyrimidines of RNA
Cytosine
Uracil
Sugar of RNA
Ribose
Sugar of DNA
Deoxyribose
- crush cell wall
Blender
Homogenizing solution
Salt and Dishwashing liquid
- removes the lipids and push DNA to the salt solution
dishwashing liquid
- increases separation of DNA from hydrophobic layer; aggregates DNA
sodium chloride
– promotes aggregation of DNA and wash the DNA from the salt
cold ethanol
Test for the deoxyribose in DNA
Dische Diphenylamine Test
Dische Test: Reagents
diphenylamine
Sulfuric acid
Dische test positive results
light blue to blue depending on the amount of DNA
Dische Test: GLUCOSE
Grayish Brown
Dische Test: RIBOSE
Greenish brown
Dische Test: DEOXYRIBOSE
Bright Blue
Dische Test: EXTRACTED DNA
Brownish Blue
Hydrolysis of DNA molecule
Acid Hydrolysis
breakdown of glycosidic bonds only if pH > 3
Depurination
Depurination
breakdown of glycosidic bonds only if pH is
pH > 3
More than 3
Products of Depurination
purine base
deoxyribose with phosphate group
breakdown of glycosidic bond with phosphodiester bond if pH < 2
Total breakdown
Total breakdown
breakdown of glycosidic bond with phosphodiester bond if pH is
pH < 2
Less than 2
Extremely low pH_____ DNA completely
hydrolyses
Products of total breakdown
phosphate group
purine or pyrimidine base
deoxyribose