Exp. 1_Proteins

Question 1

is the most abundant substance in the cell next to water, comprising ____ of its
over-all mass.

Answer 1

Protein
15%

Question 2

Protein is composed of ________as its building block.

Answer 2

amino acids

Question 3

It is linked together with _______ with a positive charged ____-containing group at one end and a negatively charged ______-group.

Answer 3

peptide bonds
nitrogen +
carboxyl-

Question 4

Along the chain is a series of different ________ from different amino acids. Some _______ are neutral, some are acidic, some are basic, and some are classified as polar or nonpolar.

Answer 4

side chains

Question 5

The different tests in this experiment will help you identify the different types of _______ present in a protein sample.

Answer 5

amino acids

Question 6

Are the tests quantitative or qualitative?

Answer 6

Qualitative

Question 7

What are the 5 tests to detect the proteins/ amino acids?

Answer 7

Biuret Test
Ninhydrin Test
Hopkins-Cole Test
Sakaguchi Test
Xanthoproteic Test

Question 8

What were the proteins used?

Answer 8

Aspartame or Equal (5 tabs or 1 sachet)
Glutathione (1 tab)
1 fresh chicken egg

Question 9

SAMPLE PREPARATION
1. Aspartame or Equal

Answer 9

1 sachet in 10 ml water

Question 10

SAMPLE PREPARATION
2. Glutathione capsule

Answer 10

Dissolve 1 tab in 5ml water

Question 11

SAMPLE PREPARATION
3. Egg Albumin Solution

Answer 11

Mix 5 ml of egg white in 45ml water