Exp. 1_Proteins| Reagent & Color Flashcards by Kyra Catedral | Brainscape

Q

Biuret Test: Reagent

A

10% sodium hydroxide solution (NaOH)

1% Copper sulfate (CuSO4)

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Q

Biuret Test: Positive Indicator

A

PURPLE color

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Q

Ninhydrin Test: Reagent

A

8% ninhydrin solution

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Q

Ninhydrin Test: Positive Detector

A

DEEP PURPLE/ BLUE color

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Q

Ninhydrin Test: Poline Present

A

Yellow

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Q

Hopkins-Cole Test: Reagent

A

Hopkins-Cole Reagent

Conc. Sulfuric acid (H₂SO₄)

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Q

Hopkins-Cole Test: Positive Indicator

A

PURPLE-COLORED RING

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Q

Sakaguchi Test: Reagent

A

10% sodium hydroxide (NaOH)

0.02% α-naphthol solution

sodium hypochlorite (NaOCl)

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Q

Sakaguchi Test: Positive Indicator

A

Red

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Q

Xanthoprtheic Test: Reagent

A

Conc. Nitric acid (HNO₃)

50% sodium hydroxide (NaOH)

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Q

Xanthoprtheic Test: Positive Indicator

A

DARK YELLOW or ORANGE color

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Q

Biuret test: detects

A

Peptide bonds/ proteins

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Q

Ninhydrin test: detects

A

Ammonia
Amines
a-amino acids

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Q

Hopkins-Cole test: detects

A

Indole Ring
Tryptophan in proteins

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Q

Sakaguchi test: detects

A

Amino acids/ proteins
Ariginine
Guanidium

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Q

Xanthoproteic test: detects

A

Benzene rings or Aromatic groups
Phenylalanine
Tyrosine
Tryptophan