Exp. 2_ Protein Denaturation Flashcards
- is the modification in conformation of protein accompanied by disruption and possible destruction of secondary, tertiary, and quaternary structure of protein.
Protein denaturation
This is brought upon by different types of agents like heat, mechanical disturbance, inorganic, and organic substances and etc.
Protein denaturation
= the frequent consequence of protein denaturation.
Loss of solubility in water
= occurs when denatured protein precipitates out
Coagulation
Denaturing Agents (based only on the conducted experiment)
- Heat
- Alcohol
- Strong Acid & Strong Base
- Heavy Metals
- Alkaloidal Reagents
In EFECT OF HEAT: egg albumin solution is heated in boiling water for how many minutes?
5 mins
In EFFECT OF ALCOHOL: what were the 2 types of reagents used?
5 ml of 95% ethanol
5 ml of 70% ethanol
In EFFECT OF ALCOHOL: what should be observed?
no precipitation
slight precipitation
heavy precipitation
In the EFFECT OF STRONG ACID & BASES: what were the 2 reagents used?
1 ml of 50% NaOH
1 ml of concentrated HNO3
In the EFFECT OF HEAVY METALS: what should be noted or observed?
Color of precipitate
Solubility of precipitate
In the EFFECT OF ALKALOIDAL REAGENTS: what were the reagents used?
1 ml of 1% picric acid solution
1 ml of 1% tannic acid solution
In the EFFECT OF ALKALOIDAL REAGENTS: what should be observed?
Color of precipitate
Protein solution needed to observe the effect of denaturing reagents
Egg Albumin Solution
TRUE/FALSE: (EFFECT OF STRONG ACID & BASE)
After adding the following reagents (50% NaOH and conc. HNO3) per albumin solution, you should shake the test tubes vigorously
FALSE; You should MIX THOROUGHLY, not shake
TRUE/FALSE:
The strong base used in the experiment is 1.5 mL of 30% sodium hydroxide
FALSE; 1mL of 50% NaOH
