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BioChem Lab > Exp. 5 > Flashcards

Exp. 5 Flashcards

1
Q

(if sample is plant) – done by mechanical disruption through crushing or blending

A

Breaking of cell wall

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2
Q

Breaking of cell membrane to expose organelles – done by adding the____________ which is an amphipathic substance

A

dishwashing liquid

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3
Q

The long hydrocarbon tail of ________ breaks the lipid bilayer of cell membrane while the _______ polar head is attracted to the water

A

soap

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4
Q

binds to the negatively charges phosphate end of the DNA chain to protect the chain as well as increases the separation of DNA from the hydrophobic layer

A

Salt

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5
Q

removes cellular debris from the sample

A

Filtration

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6
Q

Precipitation with cold alcohol

▪ DNA becomes_______ in water in the presence of salt and alcohol (more polar)

A

insoluble

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7
Q

DNA is more soluble to______ than_____ (because it is more polar),

A

alcohol

water

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8
Q

Sugar + Nitrogen Base

A

Nucleoside

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9
Q

Bonds between sugar ang base

A

Glycosidic bonds

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10
Q

Bonds between sugar + phosphate

A

Phosphodiester bonds

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11
Q
  • removes the lipids and push DNA to the salt solution
A

dishwashing liquid

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12
Q
  • increases separation of negatively of DNA from hydrophobic layer; aggregates DNA
A

sodium chloride

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13
Q

– promotes aggregation of DNA and wash the DNA from the salt

A

Add cold ethanol

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14
Q

Nucleotide is composed of

A

base
sugar
phosphate group

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15
Q

joins the base and the sugar

A

Glycosidic bond

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16
Q

joins the phosphate and sugar group

A

phosphodiester bond

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17
Q

The nucleotide chain is joined together by

A

phosphodiester bond

18
Q

break down of glycosidic bonds only if pH ______

A

> 3

Depurination

19
Q

Products are purine base and deoxyribose with phosphate group

A

Depurination

20
Q

break down of glycosidic bond with phosphodiester bond if pH____ plus____

A

< 2 + heat

Total breakdown

21
Q

Extremely low pH hydrolyses DNA________

A

completely

22
Q

Products are phosphate group, the purine or pyrimidine base, and deoxyribose

A

Total breakdown

23
Q

T or F | it is easy to attack the glycosidic bond than the phosphodiester bond

A

True!

24
Q

Test for the deoxyribose in DNA

A

Dische Diphenylamine Test

25
Q

Dische Diphenylamine Test reagents

A

diphenylamine, Sulfuric acid (2)

26
Q

Dische Diphenylamine Test positive results

A

light blue to blue solution depending on the amount of DNA

27
Q

Dische Diphenylamine Test

HYDROLYZED
UNHYDROLYZED

A

BRIGHT BLUE (+)

BROWNISH BLUE (+)

28
Q

Test for reducing sugar

A

FEHLING’S Test

29
Q

Reagents:

Fehling’s A:
Fehling’s B:

A

Copper sulfate solution

aqueous potassium sodium tartrate sodium hydroxide

30
Q

Positive result: formation of brick red precipitate

A

FEHLING’S Test

31
Q

is more sensitive to RIBOSE

Test for pentoses

A

Bial’s Test

32
Q

Bial’s test reagents

A

Orcinol
hydrochloric acid
FeCl3

33
Q

Bial’s test Positive result

A

Blue or green solution

34
Q

Test for the presence of Adenine and Guanine

A

Test for Purine Bases

35
Q

will tell if successfully na remove ang purine (AG) during hydrolysis

A

Test for Purine Bases

36
Q

Test for Purine Bases reagents

A

Silver Nitrate
Ammonium Hydroxide

37
Q

In test for purine bases, _______ in ammoniacal solution precipitates purine bases reacting to the nitrogen of purine base

A

Silver ion

38
Q

Positive result: gelatinous white precipitate

A

Test for Purine Bases

39
Q

Phosphate Test reagents

A

Ammonium hydroxide
nitric acid
ammonium molybdate

40
Q

Positive result: Yellow precipitate

A

Phosphate Test

41
Q

(NH4)3PMo12O40 6H2O

A

Ammonium phosphomolybdate ____

BioChem Lab (17 decks)

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