Exp. 3_

Question 1

PEPSIN

Answer 1

a. 5 ml distilled water
b. 5 ml 1% pepsin solution
c. 4 ml 1% pepsin solution + 1 ml 0.4% HCI solution

Question 2

In the pepsin exp. What substance was tested?

Answer 2

Meat or
Egg white

Question 3

Pepsin: Warm the test tubes in a water bath (_____ temp.) for 1 hour to 1 ½ hour. Shake occasionally.

Answer 3

37-40°C

Question 4

What does amylase breakdown?

Answer 4

Starch

Question 5

What is used to detect for the presence of starch?

Answer 5

Iodine

Question 6

Amylase: Mix both thoroughly and place in a water bath set for ____ for _____ mins.
(Make sure temperature does not raise more than 40°C)

Answer 6

37°C

15 minutes.

Question 7

What is observed in the exp. Catalase?

Answer 7

Potato

Question 8

Catalase: To the first test tube add 1ml of ______ and to the second test tube 1ml of _______. Leave the third test tube as is.

Answer 8

3% acetic acid

3% sodium bicarbonate

Question 9

Catalase: Add 3ml of 3% hydrogen peroxide to the third test tube and wait for_____

Answer 9

one minute.

Question 10

What is the substrate of PEPSIN?

Answer 10

Protein - egg white

Question 11

What is the substrate for AMYLASE?

Answer 11

Carbohydrates- starch

Question 12

What is the substrate for CATALASE?

Answer 12

Hydrogen Peroxide - H2O2

Question 13

What is the substrate fir CATALASE?

Answer 13

Hydrogen Peroxide- H2O2

Question 14

What is the substrate fir CATALASE?

Answer 14

Hydrogen Peroxide- H2O2