Exercise 22

Question 1

Kirby Bauer plate components

Answer 1

Made with mueller-hinton agar
casein ( small peptides and AAs)
Agar
Beef extract
Starch
CABS

Question 2

Media classification

Answer 2

Complex, enriched

Question 3

4 bacteria used

Answer 3

PEBS
Pseudomonas aeruginosa
E. coli
Bacillus cereus
S. aureus

Question 4

AM

Answer 4

Ampicillin, broad spectrum, blocks cell wall synthesis and transpeptidase

Question 5

Penicillin

Answer 5

Use older version, narrow spectrum against gram-positive, block cell wall synthesis/transpeptidase

Question 6

C

Answer 6

Chloramphenicol, broad spectrum, 50s ribosome blocks protein synthesis

Question 7

S

Answer 7

Streptomycin, broad, targets 30s ribosome to block protein synthesis

Question 8

TE

Answer 8

Tetracycline, broad, 30s ribosome subunit

Question 9

NB

Answer 9

Novobiocin, narrow against gram positive, targets gyrase blocking DNA synthesis

Question 10

PB

Answer 10

Polymyxin B, narrow against gram negative, targets LPS in membrane

Question 11

SXT

Answer 11

Sulfamethoxazole trimethoprim, broad, targets metabolic pathway for folic acid synthesis
UTI treatment