Exam 2 Part 3 Flashcards
This can bypass the next carrier in the chain and transfer electrons directly to oxygen
Flavoprotein
What are toxic because they oxidize biochemicals and make them nonfunctional
Hydrogen peroxide and superoxide radical
This catalyzes conversion of superoxide radicals to hydrogen peroxide
Superoxide dismutase
This converts hydrogen peroxide into water and gaseous oxygen
Catalase
What can bacteria that produce catalase he detected by
Hydrogen peroxide
What is present in a strict aerobe
Superoxide dismutase
And catalase
What is present in facultative anaerobe
Superoxide dismutase and catalase
What is present in microaerophiles
Superoxide dismutase and catalase in small amounts
What is present in aerotolerant anaerobes
Superoxide dismutase
What is present in strict anaerobes
Nothing
What is the job of the electron transport chain
Convert the coenzymes back to their oxidized state
What are the two functions of the ETC
Transport electrons down chain
Generate a proton motive force by pumping hydrogen
What are important ETC molecules unable to donate protons
Flavoproteins, iron sulfur proteins and cytochromes
What is the oxidase test designed for
Identify the presence of cytochrome c oxidase
What is the chromogenic reducing agent
Tetramethyl p phenylenediamine
What does dark blue purple color mean
Cytochrome c oxidase is present (positive)
What does no color change in the oxidase test mean
Cytochrome c oxidase is not present (negative)
What do many gram negative bacteria contain and do
Nitrate reductase and perform a single step reduction of nitrate to nitrite
This is a multi step process that enzymatically converts nitrate to molecular nitrogen
Denitrification
What does a red color after reagents A and B mean
Nitrate reduction to nitrite
What is no color change after reagents A and B
Incomplete (add zinc dust)
What is no color change after zig dust is added
Nitrate reduction to nitrogenous
What is red color after zinc dust
No nitrate reduction
