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Microbiology Lab > Exam 2 Part 3 > Flashcards

Exam 2 Part 3 Flashcards

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Biology 101 flashcards
1
Q

This can bypass the next carrier in the chain and transfer electrons directly to oxygen

A

Flavoprotein

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2
Q

What are toxic because they oxidize biochemicals and make them nonfunctional

A

Hydrogen peroxide and superoxide radical

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3
Q

This catalyzes conversion of superoxide radicals to hydrogen peroxide

A

Superoxide dismutase

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4
Q

This converts hydrogen peroxide into water and gaseous oxygen

A

Catalase

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5
Q

What can bacteria that produce catalase he detected by

A

Hydrogen peroxide

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6
Q

What is present in a strict aerobe

A

Superoxide dismutase

And catalase

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7
Q

What is present in facultative anaerobe

A

Superoxide dismutase and catalase

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8
Q

What is present in microaerophiles

A

Superoxide dismutase and catalase in small amounts

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9
Q

What is present in aerotolerant anaerobes

A

Superoxide dismutase

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10
Q

What is present in strict anaerobes

A

Nothing

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11
Q

What is the job of the electron transport chain

A

Convert the coenzymes back to their oxidized state

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12
Q

What are the two functions of the ETC

A

Transport electrons down chain

Generate a proton motive force by pumping hydrogen

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13
Q

What are important ETC molecules unable to donate protons

A

Flavoproteins, iron sulfur proteins and cytochromes

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14
Q

What is the oxidase test designed for

A

Identify the presence of cytochrome c oxidase

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15
Q

What is the chromogenic reducing agent

A

Tetramethyl p phenylenediamine

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16
Q

What does dark blue purple color mean

A

Cytochrome c oxidase is present (positive)

17
Q

What does no color change in the oxidase test mean

A

Cytochrome c oxidase is not present (negative)

18
Q

What do many gram negative bacteria contain and do

A

Nitrate reductase and perform a single step reduction of nitrate to nitrite

19
Q

This is a multi step process that enzymatically converts nitrate to molecular nitrogen

A

Denitrification

20
Q

What does a red color after reagents A and B mean

A

Nitrate reduction to nitrite

21
Q

What is no color change after reagents A and B

A

Incomplete (add zinc dust)

22
Q

What is no color change after zig dust is added

A

Nitrate reduction to nitrogenous

23
Q

What is red color after zinc dust

A

No nitrate reduction

Microbiology Lab (25 decks)

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