3-1 PL Quiz Flashcards
produces an image made from light that is transmitted through a specimen
bright field microscopy
what are most biological specimens
transparent
how is the transparency of the specimen improved
application of stains to the specimen
what is the price of improved contrast of specimen
staining process kills cells
how does image formation begin
with light coming from an internal or external light source
concentrates the light and makes illumination of the specimen more uniform
condenser lens
another name for bending of light
refraction
when is the real image produced
during the refraction of light as it passes though the objective lens from the specimen
what appears below or within the microscope
virtual image
how is the total magnification of the specimen calculated
magnification by objective lens x magnification of ocular lens
what is the practical limit to magnification with a light microscope
1300x
what is clarity of an image called
resolution
another name for limit of resolution
resolving power
this is an actual measurement of how far apart two points must be for the microscope to view them as being separate
limit of resolution
resolution _______ as the resolution is made ______
improves;smaller