3-1 PL Quiz Flashcards

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1
Q

produces an image made from light that is transmitted through a specimen

A

bright field microscopy

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2
Q

what are most biological specimens

A

transparent

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3
Q

how is the transparency of the specimen improved

A

application of stains to the specimen

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4
Q

what is the price of improved contrast of specimen

A

staining process kills cells

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5
Q

how does image formation begin

A

with light coming from an internal or external light source

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6
Q

concentrates the light and makes illumination of the specimen more uniform

A

condenser lens

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7
Q

another name for bending of light

A

refraction

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8
Q

when is the real image produced

A

during the refraction of light as it passes though the objective lens from the specimen

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9
Q

what appears below or within the microscope

A

virtual image

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10
Q

how is the total magnification of the specimen calculated

A

magnification by objective lens x magnification of ocular lens

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11
Q

what is the practical limit to magnification with a light microscope

A

1300x

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12
Q

what is clarity of an image called

A

resolution

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13
Q

another name for limit of resolution

A

resolving power

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14
Q

this is an actual measurement of how far apart two points must be for the microscope to view them as being separate

A

limit of resolution

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15
Q

resolution _______ as the resolution is made ______

A

improves;smaller

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16
Q

the best limit of resolution achieved by a light microscope is

A

.2 um

17
Q

for a specific microscope the actual limit of resolution can be calculated by using

A

wavelength/ NA condenser + NA objective

18
Q

the measure of a lens’s ability to capture light coming from the specimen and use it to make the image

A

numerical aperture

19
Q

using what increases the numerical aperture and makes its limit of resolution smaller

A

immersion oil

20
Q

a special condenser is used so only the light reflected off the specimen enters the objective

A

dark field microscopy

21
Q

uses special optical components to exploit subtle differences in the refractive indices of water to produce contrast

A

phase contrast microscopy

22
Q

light waves that are out of place will produce what

A

a degree of cancellation or darkness

23
Q

uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet radiation

A

fluorescence microscopy