3-6 PL Quiz

Question 1

what uses a dye solution in which the chromogen is acidic and carries a negative charge

Answer 1

negative staining technique

Question 2

what does an acidic chromogen give up to leave it with a negative charge

Answer 2

a hydrogen ion

Question 3

why is negative staining technique used

Answer 3

to determine morphology and cellular arrangement in bacteria that are too delicate to withstand heat fixing

Question 4

what does a negative stain produce

Answer 4

minimal shrinkage

Question 5

these are composed of mucoid polysaccharides or polypeptides that repel most stains because of their neutral charge

Answer 5

capsules

Question 6

where does the capsule stain technique stain

Answer 6

around the cells

Question 7

in a capsule stain what are the cells spread with

Answer 7

a film with an acidic stain and are not heat fixed

Question 8

what does heat fixing cause

Answer 8

the cells to shrink which leaves a white halo that looks like a capsule

Question 9

what does capsule production in some microbes do

Answer 9

makes them less vulnerable to pagocytosis