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Microbiology Lab > 3/29 > Flashcards

3/29 Flashcards

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Biology 101 flashcards
1
Q

What is a complex, selective, and differential medium

A

Eosin methylene blue agar

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2
Q

What contains a digest of gelatin, lactose, and the dues eosin and methylene blue

A

Eosin methylene blue agar

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3
Q

What is the purpose of the two dues in EMB

A

1) inhibit growth of most gram positive organisms

2) react with lactose fermenters whose acid products turn the growth black

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4
Q

What do lactose non fermenters appear like

A

Retain their normal color or take the coloration of the medium

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5
Q

What is EMB agar used for

A

Isolation of fecal coliforms

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6
Q

Fermentation of lactose by the coliform produces what

A

Shiny green appearance to the growth

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7
Q

Poor or no growth in EMB is

A

Gram positive

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8
Q

Good growth on EMB is

A

Gram negative

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9
Q

Growth is pink in EMB

A

Possible coliform

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10
Q

Growth is dark in EMB

A

Probable coliform

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11
Q

Growth is colorless in EMB

A

Noncoliform

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12
Q

What does Columbia CNA do

A

Allows growth of gram positive organisms and inhibits growth of gram negative organisms

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13
Q

What act as selective agents against gram negative organisms in Columbia CNA

A

Antibiotics colistin and nalidixic acid

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14
Q

What does the sheep blood part of Columbia CNA do

A

Differentiation of gram positive organisms based on hemolytic reaction

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15
Q

What is Columbia CNA agar used to do

A

Isolate staphylococci, streptococci, and enterococci

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16
Q

What are the only organisms that grow well on the Columbia CNA agar

A

Gram positive

17
Q

What does the NA plate will serve as what

A

A comparison for growth quality on the Columbia CNA with 5% sheep blood agar plate

18
Q

Poor or no growth on Columbia CNA

A

Probable gram negative organism

19
Q

Good growth with clearing of medium in Columbia CNA

A

B hemolytic

20
Q

Good growth with greening of medium in Columbia CNA

A

Probable ~ hemolytic

21
Q

Good growth with. No change of medium in Columbia CNA

A

Probable y hemolytic

22
Q

What does mannitol salt agar contain

A

The carbohydrate mannitol, 7.5% sodium chloride, and the pH indicator phenol red

23
Q

When is phenol red yellow

A

Below 6.8

24
Q

When is phenol red red

A

7.4 to 8.4

25
Q

When is phenol red pink

A

8.4 and above

26
Q

What does mannitol provide

A

Substrate for fermentation and makes the medium differential

27
Q

What thrive on mannitol salt agar

A

Staphylococci

28
Q

What does phenol red indicate

A

Whether fermentation with an acid end product has occurred

29
Q

What is mannitol salt agar used for

A

Isolation and differentiation of staphylococcus

Microbiology Lab (25 decks)

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