1-3 PL Quiz

Question 1

a medium that contains living microbes

Answer 1

culture

Question 2

if a culture contains a single species

Answer 2

pure culture

Question 3

what does aseptically mean

Answer 3

without contamination of yourself, others, the environment, the source culture, or the medium being inoculated

Question 4

how must you transfer microbes from their pure culture to a sterile medium

Answer 4

aseptically

Question 5

what is an LAI

Answer 5

laboratory acquired infection

Question 6

what is a procedure’s most important operational risk factor

Answer 6

potential to release microorganisms into the air as aerosols and droplets

Question 7

minimize _________________________

Answer 7

the potential of contamination

Question 8

be _________

Answer 8

organized

Question 9

place all ________________________

Answer 9

media test tubes in a test tube rack when not in use whether they are sterile or not

Question 10

take ______________

Answer 10

your time

Question 11

never ______________

Answer 11

hold a tube culture by its cap

Question 12

hold the __________________________________

Answer 12

inoculating loop or needle like a pencil in your dominant hand and relax

Question 13

adjust _____________________________

Answer 13

your bunsen burner so its flame has an inner and outer cone

Question 14

what are used to grow microbes when fresh cultures or large number of cells are required

Answer 14

broths

Question 15

these are generally used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks

Answer 15

agar slants

Question 16

these are typically used for obtaining isolation of species, differential testing, and quantifying bacterial densities

Answer 16

plated media

Question 17

the instruments used for transfers are

Answer 17

inoculating loops and inoculating needles

Question 18

what is the primary concern with BSL-2 organisms

Answer 18

contamination or infection due to inhalation of aerosols