ETC

Question 1

Total products of glycolysis, PDH, and Krebs?

Answer 1

4 ATP, 10 NADH, 2 FADH. (32 ATP)

Question 2

Is glycolysis faster in tumors?

Answer 2

Yes, 50x

Question 3

2 NADH shuttles into mitochondria

Expand

Answer 3

A-glycerophosphate (electrons to FADH2, inhibited by metformin)
Malate aspartate (NADH to NADH)

Question 4

How much of NADH and FADH2 energy is captured in ATP?

Answer 4

30%

Question 5

Eo

Answer 5

Higher affinity for electrons is higher number

Question 6

DeltaG for E0

Answer 6

-nF(delta)E0

E0 is always higher number minus smaller, n is electrons. 2 per NADH etc

Question 7

Where does NADH enter ETC? FADH2?

Answer 7

At complex one.

At complex two ( in IMM, succinate dehydrogenase)

Question 8

ETC pathway?

Answer 8

Complex one, complex two (. CoQ), complex three, cytochrome C, Complex 4.

Question 9

Complex 1 inhibitor?

Answer 9

Rotenone, MPP (Parkinson’s)

Question 10

Complex 3 inhibitor?

Answer 10

Antimycin

Question 11

Complex 4 inhibitor?

Answer 11

Cyanide. Fixed by nitrates, making methemoglobin which preferentially binds cyanide. (Ferric iron)

Question 12

What does the energy in ETC make?

Answer 12

Electrochemical gradient, pH gradient. Drives complex five, ATP synthase

Question 13

Hydrogens passed by complexes?

Answer 13

4, 2, 4

Question 14

How many hydrogens does ATP synthase pass per ATP?

Answer 14

3

Question 15

Which part of cytochrome c is targeted by CN?

Answer 15

A3. With ferric iron. Thiosulfide helps

Question 16

Which complex has no mitochondrial dna to code it?

Answer 16

2

Question 17

Complex five inhibitor

Answer 17

Oligomycin.

Question 18

Do ETC inhibitors inhibit V and vice versa?

Answer 18

Yes

Question 19

Where do uncouplers act?

Answer 19

Between complex four and five