Escherichia Coli

Question 1

Where is E. Coli located?

Answer 1

• human GI tract and UT

Question 2

How is E. coli trasmitted?

Answer 2

Trasmitted by:

• fecal - oral
• urethral migration
• colonization of catheters
• aspirations

Question 3

Is E. coli gram positive or negative?

Answer 3

gram negative rods

Question 4

E. coli culture:

Answer 4

urine, blood, CSF, on EMB or MacConkey agar

• grow at 45.5 *C
• indole postive

Question 5

Is E. coli a hemolytic or b hemolytic?

Answer 5

b-hemolytic

Question 6

Is E. coli catalase postive or negative

Answer 6

catalase positve

Question 7

Is E. coli oxidase positive or negative?

Answer 7

oxidase negative

Question 8

Is E. coli a facultative anaerobe or obligate aerobe?

Answer 8

facultative anaerobe

Question 9

E. coli is

Answer 9

glucose, lactose fermenter

Question 10

What are E. coli virulence factors?

Answer 10

1) fimbriae (pili): colonization factor
2) siderophore
3) adhesins
4) capsule ( K antigen)
5) flagella (H antigen)