Enzymes as Biological Catalysts Flashcards
What effect do enzymes have on position of Equilibrium?
-No effect
What can control enzymatic activity?
-Allosteric Modulators
What can distinguish Stereosiomers- Optical and Geometric?
-Enzymes
What is Transition State?
-Highest energy structure along the reaction
What is Activation Energy?
-Energy required to overcome to Reach Activated Complex
Activated Complex- Unstable arrangement
What two types of molecules do enzymes rely on?
Co-Factors
Co-Enzymes
What are Co-Enzymes?
Organic molecules
-Assist enzymes and can activate
What are tightly bound Co-Enzymes called?
-Prosthetic Groups
What are Co-Factors
- Inorganic molecules-metal ions etc.
- -Form a metal coordination centre in the centre of the enzyme
- Coordination centre-binds to ligands and donates electrons
What are two types of Co-Factors?
- ‘Apoenzyme’- Are enzymes without a Co-Factor
- ‘Holoenzyme’-Are enzymes WITH a Co-Factor. (enzyme+co-factor=holoenzyme)
what does ‘Induced fit’ mean?
This is a conformational change of the enzyme to fit better with the substrate.
What are Isozymes?
-These are isomers-different state of the same enzyme
Why can Isozymes help diagnose conditions?
- -Present in different tissues
- Isozymes can help diagnose a condition
Name a type of Isozyme
Protein Kinase
Name the three Isoforms of the Isozyme-Protein Kinase
M, B and MB
