Enzymes Flashcards
statins are _____ inhibitors
competitive
lineweaver buck noncompetitive inhibition displays _____
decreased Vmax
doubling the [enzyme] does not change the _______
Km
low Km = _____ substrate affinity
high
what does low Km mean?
you will get to 1/2 Vmax faster than a high Km
hexokinase location
tissues
glucokinase location
liver
hexokinase Km
low Km
glucokinase Km
high Km
why does glucokinase have a high Km?
only want to use glucokinase to convert glucose into glycogen stores when there is a high [glucose]
which type of MM enzyme inhibitor can be a transition state analog (like a drug - statin)
competitive inhibitor
where does the competitive inhibitor bind
E
where does the noncompetitive inhibitor bind
E & ES
where does the uncompetitive inhibitor bind
ES
what happens if the E & ES are bound to an inhibitor at the same time
it is reduced to a noncompetitive inhibition
what happens to Km and Vmax for competitive
Increase Km
No change to Vmax
what happens to Km and Vmax for noncompetitive
No change to Km
Decrease V max
what happens to Km and Vmax for uncompetitive
Decrease Km
Decrease Vmax
Causes a lower [ES]
what kind of inhibitors are slow acting cholinesterase inhibitors
uncompetitive
what kind of bond do irreversible inhibitors form
covalent (kills them)
what kind of inhibitor is aspirin?
irreversible (suicide)
what kind of inhibitor is cyclooxygenase?
irreversible (suicide)
what kind of inhibitor is penicillin?
irreversible (suicide)
what kind of inhibitor is transpeptidase?
irreversible (suicide)
