Enzymbiology Flashcards
What are enzymes,, What are some enzymes that are also RNA? What are some co-factors .
What are the 6 types of enzymes ? Just names
What is the role of oxidoredutases and Transferases ?
What is the role Hydrolase and lyases ?
What is the role of isomerase ? and ligases ?
What is the difference between transititon state complex and enzyme-substrate complex ?
What are the substrate binding models ?
What does an enzyme do to the activation barrier? How about free energy of reactions and products ? Does it change equilibrium ?
What do enzymes organize reactive groups ? What happens to uncatalysed bimolecular reactions ? Uncatalyzed unimolecular reactions ? How about catalysed reactions ?
What is the difference of the free energy of activation between catalyist and non catalyst ?
Draw the energy changes that occur during a reaction between a substrate and enzyme .
Why is there a dip ?
Bonds are beign formed, which is exothermic, that the energy released is used to decrease the energy of activation
What is the transition state?
When half of the reactant got converted
What are transition state inhibitors? What are transition state analogs ? What binds more tightly between the anaylog and the real subtrate ?
What is the role of pencicillin as a transition state inhibitors ? What does it interact with ?
Explain how allopurinol is an example of a transition state inhibitor ?
In the mechelis menton grapth, enzymes show what type of curve? While alloteric enzymes show what ??
What is the formula for V?
Where is Vmax, and KM ? What is the formula ?
What is known as michelis complex ?
Vo=
When the substrate is higher then the km, is in what power ? What does it mean with VMAX ? How about when substrate is less ?
intial rate
What does it mena when the Km is greater than another km in terms of affinity ?
In a lineweaver burk equation what is it ? How are the x axis and y axis represented ? What is the slope ?
What is the formula for a single substrate ? v=
What is Kcat ? What is Km ?
What are enzymes inhibitors ? What are irreversible inhibitors when are they used ?, and reversibile inhibitors ? What is the role of reversible inhibitors ?
name all the reversible inhibitors
What changes in a competitive inhibior between the km and Vmax ?
What is a non-competiive inhibitor, where does it bind ?
What changes between km and Vmax in a non-competitive inhibitor ?
What changes in a non-competiitve inhibitor
Draw the mechanism by which uncompetitive inhibition occurs .
What is allosteric regulation ? What type of ligand ? How is it regulated ?What type of modification is it ? is it reversible ? What are the modifications ? What is proteolysis role ?
What is ment by allosteric enzymes having copertivity ?
What is a positive allosteric enzymes and negative ? Why not confuse with line weaver
How does the VM and km compare in allosteric enzymes that are increase or decrease ?
Phosphorylation is a type of ? What does it do ? Can it be reversed ?
How do tissue regulate how much a certain protein they are making ?
What is enzyme synthesis ? What is protein degradation ?
