DNA Replication Flashcards
What charge do dna and RNA have ? What are the basic units or nucleoides that are found in dna or RNA ?
What is a nucleotide or nucleoside ?
Each nucloeotide has three parts, what are they ?
whats the difference of 2-deoxyribose vs ribose ? Whats different in the 2’ deoxy ? Which one is more stable and why ?
What are purines vs pyrimidines, ? What does the bottom nitron in 5’ member ring connect to ? Are bases charged ?
What do the bases provide ?
What is the purpose of a negetive charge ? The sugars and phosphate serve what purpose ?
What two forces help the bases stay togethere ?
van der waals and hydrophobic effect .
What is the A form of DNA versus the B form and Z form,
explain right twist vs left twist, and which leaves a zig zag
What does supercoiling do ?
Go to the order at which chromatin are organized .
What are the four tpes of rRNA molecules in Eukarotic cells ?
How about the three in prok
Which organell has simialr ribosome to prok ?
What is ment by the T and D and Pseudouridine in a tRNA ?
Whhat are the three mechanism of dna replication ?
What are the rules of replication ?
How many origin site does prok and euk and yeast have ?
Do the anti parallel strands replicate simultenuously ? is the leading strand and lagging strands 5’-3’ ?
What is 3’-5’ exonuclease ? what are some proof reading activities ? In DNA replication, what is added to the free 3’ OH group ? What is the use of a primer ?
What is the role of DNA pol 1,2,3 ?
in Eukaryotic DNA polymease, that is the fuction of A, B and Y , D
which oen makes primase ?
who does pol D work in conjunction with ?
What is the role of helicase ? Single strand binding protein ?
What is the role of topoisomerase ?
What i the fuction of the 1 and 2 DNA topoisomerase ?
What is the role of RNA primers, and primase ?
What is the role of DNA polymease ? What direction does it synthesize ?
What is the role of the replication folk ? How is maintained ?
Read the paragrapth
What is the role of DNA ligase ? What direction ?
Overview of the eukroitic replication complex
What are telomeres ? What is a problem developed in the lagging stand , what does it result ?
What is the role of telomerase ? Where does the primase bind ? What does telomere consist of in terms of bases ?
What does telomerase contain and what does it act like ?Where are telomerase kept active ? Why is it important that telomerase is in reproductive cells ?
What is the connection between cancer and telemorase ? What is a way to detect cancer ? What is the problem if telemorase was shut off ?
What is the association between telomere and aging ?
What are some examples of DNA damage that be based by mutagens ( list of mutagens)
What is a spontaneous mutations ? What is tautomerism,Depurination,Deamination,Transition,Transversion ?
What is induced mutatation ? What is one of them ?
What is base excision repair ?
What is nucleotide excision repair ?
What is mistched repair ?
The stand that is methylaton has the GATC sequnce, then MUT S finds the sequence(correct strand) that is damaged, and then MuT L binds, Then the Mut H binds to the CATC ( highly methylated site)
Then the MUt S and L interact with MUt H by lopping , which Mut H makes a hashes( cuts) and cuts the unmethylated, and endoclease cuts past the mismath strand where the Mut S and L are, and RNA poly is used
What is transcription coupled repair ?
What is Xeroderma pigmentosum ? What is it fuciton ?
What are the three types of recombination ? What ar ethe consequences of the dynamic nature of chromosome ?
What is homologous recombination ? What is holliday model ?
What is translocation ?
What is transposable elements ?
