Environmental Control of Metabolism Flashcards

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1
Q

What are microorganisms?

A

Very small, unicellular organisms.

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2
Q

What domains of life can microorganisms belong to?

A

Eukaryotes, Bacteria, and archaea.

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3
Q

Why are microorganisms useful?

A

They produce a variety of metabolites from their metabolic reactions.

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4
Q

What uses do microorganisms have in industry?

A
  • medicine
  • food and enzyme
  • bioremediation
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5
Q

Why are microorganisms ideal in industry?

A
  • easy to culture
  • reproduce and grow quickly
  • their food substrate is often cheap
  • produce useful products
  • highly adaptable
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6
Q

How can microorganisms be grown successfully in a lab?

A
  • appropriate growth medium

- controlled environmental factors

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7
Q

What domain of life are microorganisms found in?

A

All three - bacteria, archaea, and eukaryotes.

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8
Q

Where can microorganisms get their energy?

A
  • photosynthesis

- in the chemical industry, a chemical substrate

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9
Q

Where are microorganisms usually grown when used in the chemical industry?

A

On agar plants which contain nutrients.

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10
Q

Explain the difference to microorganisms and complex molecules.

A

Some can produce all the complex molecules required whereas others need this to be added to the growth media.

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11
Q

What must be controlled when growing microorganisms on a growth medium?

A
  • sterile
  • temperature
  • oxygen
  • pH
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12
Q

How do you ensure sterility?

A

Aseptic techniques, steam, and filters. Reduces competitions with desired microorganisms and reduces the risk of spoilage.

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13
Q

How do you control temperature?

A

Water jackets and a thermostat. Keeps enzymes at their optimum temperature.

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14
Q

How do you control oxygen levels?

A

Air inlets and paddles - allow aerobic respiration to occur.

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15
Q

How do you control pH levels?

A

Buffer or addition of acids/alkalis to keep enzymes at their optimum pH.

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16
Q

What pH do most bacteria grow at?

A

pH 7

17
Q

What pH do most fungi grow at?

A

pH 5-6

18
Q

What are the four stages of growth?

A
  1. lag
  2. log
  3. stationary
  4. death
19
Q

What happens in the lag phase?

A

Enzymes are induced in order to metabolise substrates.

20
Q

What happens in the log phase?

A

The most rapid growth of microorganisms due to plentiful nutrients.

21
Q

What happens in the stationary phase?

A

Occurs due to nutrients in the culture media becoming depleted and production of toxic metabolites.

22
Q

What stage are secondary metabolites (antibiotics) produced?

A

Stationary phase

23
Q

What happens in the death phase?

A

Occurs due to the toxic accumulation of metabolites or the lack of nutrients in the culture.

24
Q

What is the total cell count?

A

Total number of cells alive and dead.

25
Q

What is the viable cell count?

A

Total number of living cells