Energy systems, digestibility Flashcards
What is digestible feed?
The portion of the feed which is not excreted in feces and is assumed to be absorbed by the animal.
How is digestibility usually estimated and expressed?
Estimated in DM basis.
Expressed as a coefficient or percentage.
What is the formula for DM digestibilty?
(consumed-excreted)/consumed
What is apparent digestibility?
An estimation = feed ingested - feces excreted
What is true digestibility?
True digestibility involves correction for endogenous losses.
What information do you need to determine true digestibility?
- Sloughed off intestinal cells
- Digestive secretions (enzymes, mucus)
- Microbial matter
Which is higher, true or apparent digestibility?
True is higher than apparent
Which digestibility is more difficult and costly to determine?
True digestibility.
What are the different ways to measure digestibility in vivo?
- Growth trials
- Direct (total collection)
- Difference (basal + test food)
- Regression
- Tracer or indicator
Which in vivo measurement of digestibility is the most accurate?
Direct (total collection)
What are in vitro methods of digestibility measurement?
- Digestion stimulation
- Gas production
What is the adaptation period for direct measurement of digestibility?
1-2 weeks
How is direct (total collecttion) measurement of digestibility done?
- Feed a known quantity to the animal at the same time every day.
- The amounts of food eaten should not vary from day to day.
- Collect total fecal output for 5-14 days (during diet feeding period).
- Chemical analysis of feed and feces.
- Apparent digestibility calculation.
What are the lab conditions for sheep and cattle digestibility studies?
- Metabolic crates or chambers
- Animals are fitted with strap-on canvas fecal collection bags
- Urinary catheters are placed to prevent the contamination of feces with urine
NOTE: lab conditions impact digestibility
When are tracers or indicators used?
When it is impossible to measure total intake and fecal output (ex. group feeding or grazing animals).
What are the characteristics of a tracer?
- Indigestible
- Non-absorbable
- Non-toxic
- Easy to analyze
How are tracer studies done?
- Known tracer amount and known feed amount are given.
- Collection of fecal samples for at least one week of marker feeding.
- The marker is given continuously during the trial period until it is stabilized in the feces.
What are internal markers/tracers?
- Lignin
- Acid insoluble ash (silica)
- N-alkanes
What are external markers/tracers?
- Cr2O3 (ruminants)
- TiO2 (pigs, poultry)
What are the pros of using a tracer?
- Total feces collection is not necessary.
- Total intake determination is not necessary.
- Easier, less labor.
What are the cons of using a tracer?
- Representative sampling is essential.
- Accurate estimation of nutrient or marker concentration is essential.
- It assumes complete excretion of the marker and hence the recovery of the marker determines accuracy.
What is the definition of in vitro measurement of digestibility?
Lab methods to simulate digestion by the animal.
How is in vitro measurement of digestibility used for non-ruminants?
Protein digestion is determined by digesting the feed in pepsin and HCl.
How is in vitro measurement of digestibility used for ruminants?
The feed is incubated with rumen fluid under anaerobic conditions. The bacteria are then killed by acidifying with HCl and digested in pepsin.
What temperature is rumen fluid incubated at?
39 C
How is digestibility in different GIT portions measured?
In situ - fistulated animals (surgical procedure)