EEMB 3: Microbial Biology Flashcards
What is mineralizing
conversion of organic matter to inorganic substiuents
What performs minerlization
microbes
T/F: The body consists of as many microbes as human cells
FALSE a lot more microbes
T/F: Microbes help perform bodily fucntions
True
“the invisible presence”
microbes
the life force of the planet
microbes
The origins of life are based on
First principles
Name the hypothesis of water
degassing-condensation
comets and asteroids bringing ice
The first microfossils appeared when
3.5-3.8 bya
6 essential characteristics of life
- membrane
- aqueous
- catalysts
- energy flow/storage
- anabolic pathways (polymerizing molecules)
- RNA/DNA
describe the membrane characteristic
layer separating outer environment and inner
describe aqueous
water-based organism
describe catalysts
speed up reactions
describe energy flow/storage
metabolism (ATP -> ADP Pi)
describe anabolic pathways
monomers -> macromolecules
describe RNA/DNA
info carriers/storage + translation apparatus
Name the 3 Theories of life
Gaia Hypothesis
Vital Force View
Mechanistic View
What is the Gaia Hypothesis
earth is a living entity - its biosphere maintains its homeostasis
What is the Vital Force View
science cannot explain life forces (not testable) and does not include physics and chemistry
What is the Mechanistic View
Life is chemically based with same chemicals from environment, just in a particular and unique arrangement
Can some forms of life form from inanimate matter?
Nopes
Which two guys disproved spontaneous generation
Redi and Pasteur
Redi’s exp
flies and rotten meat in a jar
Pasteurs exp
Broth and swan-neck flask
Origins Hypothesis I is
panspermia
What is panspermia
extra terrestrial life was brought here in the form of germs/spores
Origins Hypothesis II is
Chemical Evolution (life is unique to Earth)
Stages of Chemical Evolution
1) Nucleotide/Amino Acid formation
2) Polymerization
3) Polymers in membranes
4) Living
Explain the 3 ways of nucelotide/amino acid formation
E.T. input -> water and organic material delivered by comets and asteroids
Reducing atmosphere air contained CH4 NH3 CO2 H2S, H2, NOO2
Hydrothermal Vents: Chemicals interact with energy from heat (CH4 NH3)
Explain the stage of polymerization
[difficult with no enzymes present]
Energy for polymerization could be possible from:
1) pyrite
2) hydrothermal vents
3) hot pools
What is pyrite
sillicate in clay that condense to catalyze
what is a hydrothermal vent
vent that consists of metas, iron and nickel, that could catalyze
What are hot pools
places where water evaporates and leaves more concentration of materials to interact with each other
Explain the stage of polymers in membranes
Membranes form around the polymers and the result is a protobiont
What is a protobiont?
aggregate of organic molecules that perform metabolism
Catabolism
breaking down materials
Anabolism
synthesizing materials
What are coacervates
specific protobionts that have lots of sugars and proteins
What is the coacervates relationship to the environment?
its internal chemistry is sginificantly different than the surrounding environment
The catalyst that allowed protobionts to become living
RNA ribosyme (ribosome)
Information carriers in the living microbe
RNA and (Hydrogen bonds)
T/F: Ribozyme is an enzyme
FALSE it is only made of nucleotides (amino acids) not proteins
Catabolism
breaking down materials
Anabolism
synthesizing materials
What are coacervates
specific protobionts that have lots of sugars and proteins
What is the coacervates relationship to the environment?
its internal chemistry is sginificantly different than the surrounding environment
The catalyst that allowed protobionts to become living
RNA ribosyme (ribosome)
Information carriers in the living microbe
RNA and DNA
T/F: Ribozyme is an enzyme
FALSE it is only made of nucleotides (amino acids) not proteins
Other things that allowed microbes to be classified as living
RNA/DNA (hydrogen bonds) (translation/transcription apparatus)
Two forms of dating
relative and absolute
Relative dating
straiographic layers (sediment layers and fossils)
Absolute dating
Half-life (radioactive isotopes)
Linnaen Classification
Kingdom - Phylum - Class - Order - Family - Genus - Species (most specific)
KPCOFGS
Characteristics conserved over all 3 domains
RNA/DNA Translation/Transcription apparatus Glycolysis Ribosomes Semi-conserved DNA Replication Plasma Membranes
Which of the 3 domains is most different from the others
bacteria (archaea and eukarya most similar)
Evolutionary Distance
different base pairs/ total base pairs (presented in %)
What are all prokaryotes?
Microbes
What is the size range of prokaryotes?
100 nm to 100 micrometers
What portion of Eukaryotes are microbes? Examples?
1/3
Protists and fungi
What is the advantage prokaryotes have over multi-cellular eukaryotes?
Individual cells can survive by themselves (while other cells need to communicate)
Advantages to being small
less competition for space
smaller surface area/volume to move nutrients, excrete waste
less nutrients needed to maintain
Phytoplankton is what percent of the earths biomass? What % of the Earths oxygen do they produce?
0.2% biomass
45-5-% oxygen
What type of organism is phytoplankton?
photoautorophs
What type of organism is bacterial plankton?
heterotroph
2 other names for autotrophs, derived from what?
chemo-autotrophs
chemo lithotrophs
derived from converting inorganic materials and CO2 –> organic and oxygen
What are ribozymes?
selt-catalyzing strands of RNA that also carry information
Proteins become specific to
control reactions
RNA folding leads to
stabilization of H-bonds in 3D form
What is a reducing atmosphere?
Atmosphere created by desne gasses (volcanic out-gassing)
H2O, ClH2, CH4, H2S, NH3, CO2
Miller-Urey experiment? Did it work?
Emulated conditions of early Earth (reducing atmosphere) to try and create organic compounds.
Produced nucleotides and AAS
Nucleotides and AAs are precursors for
DNA and proteins/enzymes
Hydrothermal vents created first organic compounds through (specific terminology)
Thermal gradients and “plume chemistry”
Some factors for extinction and evolution
continental drift, climate change, volcanic activiy, unidirectional change in O2, external events.
Taxonomy
Theory and practice of classifying organisms
Phylogeny
classifying organism based on evolutionary history
Systematics
the science of studying diversity of organisms and reconstructing phylogeny
2 ways changes in living organisms happen
Genetic exchange
Environmental change
Properties of rRNA that help construct phylogenies
its a structural gene
highly conserved
sufficient variability
T/F: Not all prokaryotes are microbes
FALSE
How many entire domains are microbes?
2 (bacteria and archaea)
Domains are set based on which rRNA gene sequences?
16s and 18s
Characteristics of Microbes
sing cell or cluster
stand alone organisms
capable of living independent of tissue (growth, energy generation, reproduction)
Traits that set microoganisms
metabolism, reproduction, differentiation, communication, movement, evolution
Common features all microbes share
live in water
food for higher trophic levels
small
T/F: Microbes are not the most abundant organisms in the biosphere
FALSE they are bro
T/F: Microbes have a vast physiological diversity (functions)
True
2 physiologies of Macrobes
photosynthesis (chloroplasts)
heterotroph (mitochondria)
2 basic metabolic strategies
photosynthesis
respiration
Another name for photosynthesis and formula. Example?
photoautotrophy
CO2 + nutrients –> organic matter + O2
Phytoplankton
Another name for respiration and formula. Example
Heterotrophy
organic matter + O2 -> CO2 + nutrients
Bacterioplankton
What is the base of the food web at Hydrothermal vents?
Sulfur oxidizing bacteria
H2S + 2O2 –> SO4 + 2H
The element most associated with mircobes
nitrogen
Macro nutrients
C,H,O,N,P,S
T/F: Cells are at equilibrium
FALSE, they are non-equilibrium systems
5 Huge impacts of microbes
Majority of earths biomass Various functionality Biogeochemical agents Control human history Industry
Advances in microbiology come from
technological develoments
Most early microbe work focused on aspects related to
humans
How old of a science is microbiology?
young science
Who created the first microscope
Leeuwenhoek
What did he call the first observed bacteria
animicules
What is a major technological development in the microbiology world?
optics
2 terms of microscopy?
magnification
resolution
Magnification does what?
increase the viewing size ONLY
Resolution does what
distance between two points, used to gain information