Dx Of Viral Infections

Question 1

What are the 7 different ways to Dx viral infections?

Answer 1

1. PRC
2. Real time or Q-PCR
3. Genome sequencing
4. Next generation sequencing
5. Metagenomics
6. Phylogenetic analysis
7. Microarrays

Question 2

What does PCR do?

Answer 2

Amplify viral genome/DNA

Question 3

What are the 3 steps of PCR?

Answer 3

Denaturation
Annealing
Extension/elongation

Question 4

What is real-time PCR or Q-PCR?

Answer 4

Monitors and quantifies by increasing accumulation of PCR products/nucleic acid loads (useful to study virus load in patient)

Question 5

The process by which the sequence of bases in a DNA molecule is elucidated/can be obtained and read is called what?

Answer 5

DNA sequencing

Question 6

Which sequencing is significantly cheaper, quicker, needs less DNA, has high throughput, and is more accurate and reliable than Sanger sequencing?

Answer 6

Next generation sequencing

Question 7

What is metagenomics?

Answer 7

The study of the collective set of microbial populations in a sample by analyzing the sample’s entire nucleotide sequence content

A powerful method for random detection of existing and new pathogens

Question 8

The study of virus evolution using genome sequencing data is known as what?

Answer 8

Phylogenetics

Question 9

What kind of assay places several thousands of known DNAs amplified via PRCs/RT-PCRs on a glass or silicon cup in order to detect target DNA?

Answer 9

Microarrays

Question 10

What is the advantage of microarrays?

Answer 10

Hundreds of pathogens can be screened for simultaneously using a single microarray chip