DNA, RNA, Protein Synthesis Flashcards
Nucleic Acid
Polymers of nucleotides.
Function of DNA
provides code for cell’s activities
Function of RNA
converts code into proteins
Parts of a nucleotide
1 pentose sugar, 1 nitrogenous base, 1 phosphate group
Nitrogenous Bases
adenine and thymine or uracil
guanine and cytosine
Pyridimines
cytosine, thymine, uracil
have a single ring structure
purines
adenine and guanine
have a double ring structure
Hydrogen Bonds Between Nitrogenous Bases
adenine and thymine/uracil form 2 hydrogen bonds
guanine and cytosine form 3 hydrogen bonds
ATP (Adenosine Triphosphate) Function
an energy source for cellular activities
ATP Components
adenine base, ribose sugar, 3 phosphate groups
Formation of ADP & ATP
when a phosphate group is removed from atp, energy is released and ADP forms.
when energy is absorbed by ADP and a phosphate group bonds to ADP, ATP is formed.
3 DNA Functions
replicates itself, mutates, aids in control of cell activities (involved in protein synthsis, proteins carry out activities)
DNA Replication
- two strands of DNA “unzip” (hydrogen bonds broken by helicase)
- nucleotides move to position by way of complementary base pairing (A-T, G-C)
- adjacent nucleotides become joined together by DNA polymerase
- 2 identical DNA molecules produced
Types of RNA
- Messenger (mRNA): copy of DNA
- Ribosomal (rRNA): docking site fo mRNA
- Transfer (tRNA): “taxi” of amino acids
Gene Expression
The synthsis of a protein from a gene sequence
Processes of Gene Expression
Transcription and Translation
Transcrition
In the nucleus, DNA double helix is split (HB broken), RNA polymerase reads DNA and constructs mRNA
Translation
Occurs in cytoplasm, mRNA is translated into a protein.
Translation Steps
**1. Initiation **(ribosome assembles near start codon on mRNA (AUG), and tRNA brings anticodon with amino acid methionine
2. Elongation (ribosome moves along mRNA, tRNA brings amino acids and they are added to polypeptide chain)
3. Termination (stop codon enters ribosome, polypeptide chain is released)
Mutations
Permanent change in dna base sequences
Mutagens
environmental factors (radiation, chemicals) that damage dna
Point Mutation
Affects only one base pair
Types of Point Mutations
- silent (no change)
- missense (change in aa sequence)
- nonsense (stop codon)
Frameshift Mutation
one or more nucleotides inserted or deleted, results in shift of reading frame or insertion of a stop codon
Cell Theory
The basic theory of biology that states:
- all living things are made of cells
- all cells come from pre-existing cells
- the cell is the smallest unit of life