Disposal of N

Question 1

Pro sequence

Answer 1

part of native protein

• blocks the catalytic site
• remove this by proteolysis

activation of zymogens

Question 2

Chymotryypsin vs trypsin vs elastase

Answer 2

They all result in free amino acids

chymo-bulky hydrophobic AA

Trypsin-basic AA

Elastase-Aliphatic AA

Question 3

Urease

Answer 3

high levels of urea in circulation can go into the intestine-get broken down by bacterial enzyme urease-release ammonia-back into circulation-TOX

Question 4

B6 role in amino transferase

Answer 4

takes NH2 from alpha amino acid and puts onto glutamate

Question 5

the three important AA/Keto acid pairs

Answer 5

glutamate transamination alpha-ketoglutarate

alanaine (glucose alanine cycle) pyruvvate

Asparate (urea cycyle) oxaloacetate

All second things are TCA intermediates
-when low on these-start breaking down protein

Question 6

alanine amino transferase

Answer 6

Pyrvuate to alanineNH3 to take NH3 from glutamate

Happens in SM

Sequester NH3-move to liver, take off NH3 and regen pyruvate, then make it glucose

Question 7

Ammonia transport

Answer 7

Can not be transported

Unless assimmilated in glutamine, aspargine, or alanine

Question 8

Glutamine

Answer 8

In all tissues

Sudden rise in ammonia levels-sequestering of ammonia

Requirres ATP

Question 9

L alanine vs pyruvate

Answer 9

L alanine is pyruvate with NH3

Question 10

Glutamate vs alpha ketoglutrate

Answer 10

alpha is glutamate without NH3

Question 11

Glutamate vs glutamine

Answer 11

Glutamine is glutamate with extra NH2