Different DNA and RNA polymerases

Question 1

DNA III polymerase

Answer 1

prokaryotes

Leading and lagging strands
Has 5’–>3’ synthesis and
3’–>5”exonuclease activity

Question 2

DNA I polymerase

Answer 2

Prokaryotes

Excises RNA primer
-5’ –> 3’ exonuclease activity along with 5’–>3’ DNA synthesis and proof reading 3’–> 5’

Question 3

DNA polymerase alpha

Answer 3

Eukaryotes

RNA primer and builds okazaki fragments on lagging strand

Question 4

DNA polymerase beta

Answer 4

Eukaryotes

DNA repair

Question 5

DNA polymerase Gamma

Answer 5

Eukaryote

LEADING STRAND

Question 6

DNA polymerase gamma

Answer 6

mitochondrial DNA

Question 7

RNA polymerase I

Answer 7

rRNA

is synthesized in nucleolus

Question 8

RNA polymerase II

Answer 8

mRNA

Question 9

RNA Polymerase III

Answer 9

tRNA

Question 10

alpha- amanitin

Answer 10

Found in Amanita Phalloides (death cap mushrooms) and it inhibits RNA polymerase II. Can cause severe hepatotoxicity if ingested

Question 11

Rifampin

Answer 11

inhibits RNA polymerase in prokaryotes

(recall that prokaryotes have only one type of RNA polymerase and it is a multisubunit complex that makes all 3 kinds of RNA)

Question 12

Actinomycin D

Answer 12

inhibits RNA polymerase in both prokaryotes and eukaryotes