Diagnostic immunology

Question 1

Define monoclonal antibody

Answer 1

an antibody produced by a single clone of cells and consist of identical anitoby molecules

Question 2

define hybridoma and their use

Answer 2

• fusion of cultured myeloma (cancerous B cells) with antibody producing spleen cells
• Use: can be used to culture large amounts of identical antibodies (monoclonal)

Question 3

what is Serology

Answer 3

use of serum antibodies to detect and measure antigens or vice versa

evaluates antibody and antigen interaction

Question 4

define Immunoassay

Answer 4

assays or test which use immunological reagents like antibodies and antigens

Question 5

define seroconversion

Question 6

what are the serological test that use antigens and corresponding antibodies

Answer 6

• precipitation test
• agglutination test
• neutralization test
• complement fixation test
• labeled antibody test

Question 7

what are the essential elements of a diagnostic test? (definition too)

Answer 7

• Sensitivity: probability that test is reactive if the specimen is a true positive
• specificity: the probability the test will not be reactive if the specimens is a true negative